The reconstituted protease inhibitor solution may be stored at 2-8 °C for at least 2 weeks. Long term solution stability studies at -20°C have not been performed, however 10X solutions - 10mL per tablet - will be stable for at least 1 month in frozen aliquots. Avoid repeated freeze/thaw cycles.
推薦產品
產品名稱
SIGMAFAST ™蛋白酶抑制剂混合物片剂，无EDTA, for use in purification of Histidine-tagged proteins
生物源
bovine lung
品質等級
形狀
tablet
儲存溫度
2-8°C
尋找類似的產品？ 前往 產品比較指南
一般說明
SIGMAFAST™ 蛋白酶抑制剂混合液片，EDTA-Free是一种水溶性片剂配方，旨在抑制丝氨酸、半胱氨酸、天冬氨酸和金属蛋白酶。 该片剂不含强金属螯合剂，如EDTA，并与固定化金属亲和层析（IMAC）样品处理兼容。 该片剂还含有独特的专利配方，包括胃酶抑素A盐，以提供额外的保护。
特異性
抑制丝氨酸、半胱氨酸、天冬氨酸和金属蛋白酶。
應用
SIGMAFAST ™蛋白酶抑制剂混合液片的广泛特异性 使其适合用于组氨酸标记蛋白的纯化， 并作为通用蛋白酶抑制剂混合液。 该片剂还可以用作细胞裂解缓冲液的组件， 并已与HIS-Select介质一起使用进行了优化和测试。
特點和優勢
EDTA-Free： 不含可能干扰IMAC处理的强金属螯合剂，如EDTA。
胃酶抑素A：含有独特的专有配方（包括胃抑素A盐），可提供片剂形式通常无法提供的额外保护作用。
广泛特异性：抑制丝氨酸、半胱氨酸、天冬氨酸和金属蛋白酶，为蛋白质样品提供广泛的保护。
高产率：一片可制成100 mL混合液，推荐用于抑制100 mL提取物中最多20 g细胞质量中存在的蛋白酶。
长期稳定性：该片剂在2–8 °C温度下至少可稳定保存2年。复溶的蛋白酶抑制剂溶液（1´或10´）在2–8 °C下至少可稳定保存2周。
胃酶抑素A：含有独特的专有配方（包括胃抑素A盐），可提供片剂形式通常无法提供的额外保护作用。
广泛特异性：抑制丝氨酸、半胱氨酸、天冬氨酸和金属蛋白酶，为蛋白质样品提供广泛的保护。
高产率：一片可制成100 mL混合液，推荐用于抑制100 mL提取物中最多20 g细胞质量中存在的蛋白酶。
长期稳定性：该片剂在2–8 °C温度下至少可稳定保存2年。复溶的蛋白酶抑制剂溶液（1´或10´）在2–8 °C下至少可稳定保存2周。
成分
AEBSF
乌苯美司
E-64
抑肽素A
磷阿米酮
亮抑酶肽
抑酶肽
乌苯美司
E-64
抑肽素A
磷阿米酮
亮抑酶肽
抑酶肽
數量
一片可制备100 mL混合液。 推荐将一片用于抑制100 mL提取物中最多20 g细胞质量中存在的蛋白酶。
外觀
片剂是可溶于水的
儲存和穩定性
该片剂在2-8°C温度下至少可稳定保存2年。重悬的蛋白酶抑制剂溶液（1´或10´）在2–8°C下至少可稳定保存2周。
法律資訊
HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC
也與該產品經常一起購買
相關產品
產品號碼
描述
訂價
訊號詞
Danger
危險聲明
危險分類
Eye Dam. 1 - Skin Corr. 1A
儲存類別代碼
8A - Combustible corrosive hazardous materials
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges
從最近期的版本中選擇一個：
分析證明 (COA)
Lot/Batch Number
客戶也查看了
Stephan G Mauracher et al.
Phytochemistry, 99, 14-25 (2014-01-28)
Tyrosinases catalyze two initial reaction steps in the formation of melanin. Purification of tyrosinases had always been a process accompanied with various problems caused by enzymatic browning processes. Here, an approach is presented for the purification of the latent enzyme
Katarzyna Rybak et al.
Developmental cell, 29(5), 607-620 (2014-06-03)
Plant cytokinesis is initiated in a transient membrane compartment, the cell plate, and completed by a process of maturation during which the cell plate becomes a cross wall. How the transition from juvenile to adult stages occurs is poorly understood.
Benjamin M Nitsche et al.
BMC genomics, 13, 380-380 (2012-08-10)
Filamentous fungi are confronted with changes and limitations of their carbon source during growth in their natural habitats and during industrial applications. To survive life-threatening starvation conditions, carbon from endogenous resources becomes mobilized to fuel maintenance and self-propagation. Key to
Zilun Wei et al.
Life sciences, 232, 116632-116632 (2019-07-07)
The inflammation modulation effects of mesenchymal stromal cell-derived exosomes (MSC-EXO) are well established. We aimed to explore the mechanism behind the inflammatory responses of numerous exosomal cargo molecules that have been neglected in molecular biology research, and to develop an
Jeffrey L Waugh et al.
PloS one, 6(11), e27984-e27984 (2011-12-02)
Regulator of G protein signaling 9-2 (RGS9-2) is a protein that is highly enriched in the striatum, a brain region that mediates motivation, movement and reward responses. We identified a naturally occurring 5 nucleotide deletion polymorphism in the human RGS9
文章
相關內容
Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.
根據您的需求選擇不同類型的蛋白酶抑制劑，防止蛋白質在分離和表徵過程中降解，保障樣品預備過程中蛋白質的安全。
-
Can the reconstituted protease inhibitor cocktail solution be stored at -20°C and for how long?
1 answer-
Helpful?
-
-
What is the difference between Protease Inhibitors P8849 and S8830?
1 answer-
P8849 is a DMSO solution and S8830 is a water soluble tablet. P8849 formulation is considered proprietary but does contain AEBSF, Pepstatin A, Bestatin, E-64, and Phosphoramidon. The formulation of S8830 also contains Aprotinin and Pepstatin A and the exact formulation is given on the product information sheet (under Documents, above).
Helpful?
-
-
How should I dissolve Product No. S8830, SIGMAFAST™ Protease Inhibitor?
1 answer-
You can dissolve the product in 100 ml of water to make a 1X solution. You can make a 10X stock solution using 10 ml of water and then dilute 1:10 for use. Concentrations greater than 1X may appear slightly hazy. This will not affect the performance of the protease inhibitors.
Helpful?
-
-
What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
-
-
Can Product No. P8849, Protease Inhibitor Cocktail, be used with with HIS-Select™ products or any other immobilized metal affinity chromatography (IMAC) products?
1 answer-
This product does not contain EDTA and can be used with sample processing by immobilized metal affinity chromatography (IMAC) suchas used for the purification of histidine-tagged proteins..
Helpful?
-
-
Are there publications that cite use of Product S8830, SIGMAFAST™ Protease Inhibitor Cocktail Tablets?
1 answer-
Yes. Several examples of published papers that cite the use of Product S8830 include these:1. Smith, E.R., et al., Instability of fibroblast growth factor-23 (FGF-23): Implications for clinical studies. Clinica Chimica Acta, 412(11-12), 1008-1011 (2011). Use was with blood at 20 μL cocktail per 1 mL of blood.2. Reber, S., "Isolation of Centrosomes from Cultured Cells" (Chapter 8), from Microtubule Dynamics: Methods in Molecular Biology (Springer Protocols), vol. 777, pp. 107-116 (2011).
Helpful?
-
Active Filters
我們的科學家團隊在所有研究領域都有豐富的經驗，包括生命科學、材料科學、化學合成、色譜、分析等.
聯絡技術服務
