The unit activity of this product is lot-specific and reported in the product Certificate of Analysis. The minimum activity is 250 units per microliter. The KU value represents 1000 units. For example, a 20 KU package size represents 20,000 units. Please see the link below to review a sample or lot-specific Certificate:
https://www.sigmaaldrich.com/product/sigma/e1014#product-documentation
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生物源
Serratia marcescens
品質等級
重組細胞
expressed in E. coli
化驗
≥90% (SDS-PAGE)
形狀
buffered aqueous glycerol solution
分子量
30 kDa
濃度
≥250 units/μL
應用
research use
異物活動
protease, essentially free
運輸包裝
wet ice
儲存溫度
−20°C
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一般說明
應用
Benzonase®全能核酸酶用于：作为冰冷裂解缓冲液C的组分以消化DNA 和 RNA，便于充分释放所有核蛋白[3]在免疫沉淀中用于释放核质和染色质中的蛋白复合物[4]作为RIPA添加剂，用于分离SHSY5Y细胞进行免疫沉淀[5]在脱细胞方法中用于去除主动脉根部的残留核酸[6]
用于去除蛋白样品中的核酸。
生化／生理作用
Benzonase®全能核酸酶能将核酸完全消化成长度为3到5个碱基的5′-单磷酸末端寡核苷酸，使其成为从重组蛋白中去除核酸的理想工具，并适于需要完全消化核酸的应用。除了降低蛋白质提取物的粘度并防止细胞结团之外，使用Benzonase®全能核酸酶预处理蛋白质样本可以消除任何结合的核酸，进而显著提高2D凝胶电泳的分辨率。这种多功能酶可消化天然或热变性的DNA和RNA，其酶活性的最佳pH值为8.0-9.2。Benzonase®全能核酸酶可有效去除微生物组样本中的宿主DNA。在许多情况下，微生物组样本（如唾液或皮肤等）中含有较高比例的能够干扰下游结果的宿主DNA。我们的专家表示，减少宿主DNA可以降低测序成本，同时增加并改进数据。实验数据显示于以下技术文章中-用于微生物组工作流程的Benzonase®全能核酸酶物
消化天然或热变性的DNA和RNA。
特點和優勢
- 去除微生物组样本的宿主DNA。
- 在各种工作流程中高效去除核酸。
- 在蛋白提取过程中降低粘度。
單位定義
在pH 8.0，37 °C条件下，一个单位的酶在30分钟内可将超声的鲑鱼精子DNA消化成等同于1.0 ΔA260的酸可溶寡核苷酸（2.625 ml反应体积）。
外觀
在含有20 mM Tris HCl, pH 8.0, 2 mM MgCl2及20 mM NaCl的50%甘油中的溶液。
其他說明
法律資訊
Benzonase®核酸酶由德国达姆施塔特默克公司和/或其附属公司提供。
Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves
從最近期的版本中選擇一個：
分析證明 (COA)
Lot/Batch Number
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文章
Benzonase® Nuclease 用於減少微生物群工作流程中的宿主 DNA，並加強分類鑑定。
Benzonase® Nuclease for reducing host DNA in microbiome workflows and enhancing taxa identification.
This page lists nine frequently asked questions and answers about Benzonase® Nuclease.
本頁面列出 9 個關於 Benzonase® 核酸酶的常見問題與解答。
相關內容
The use of Benzonase® endonuclease can significantly reduce the levels of DNA by more than 100,000-fold while also reducing viscosity and protecting downstream equipment from DNA fouling. However, optimization strategies and DoE are critical when it comes to reducing DNA in your process. Setting up a DoE for your Benzonase® endonuclease application can help you find the optimal operation conditions that deliver the required DNA clearance from your process.
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How many ul does it contain? What kind of Unit is KU?
1 answer-
Helpful?
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Hi, for protein purification from E. coli cells, how much is the working concentration range for Benzonase nuclease (≥250 units/μL)? Thank you
1 answer-
The recommended starting concentration for Benzonase nuclease is 25 units per milliliter of cell lysate.
Helpful?
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What is the storage temperature range for E1014? There is only a specified storage temperature, and not a range.
1 answer-
This product is stored at freezer temperature, which is typically -20°C. An excepted range is -15 - -20°C.
Helpful?
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