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539137

Sigma-Aldrich

Protease Inhibitor Cocktail V

EDTA-Free, lyophilized, A cocktail of four protease inhibitors for the inhibition of serine, cysteine, but not metalloproteases.

同義詞:

EDTA free Protease inhibitor

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About This Item

分類程式碼代碼:
12352200
NACRES:
NA.54

product name

蛋白酶抑制剂混合物套装V,无EDTA, A cocktail of four protease inhibitors for the inhibition of serine, cysteine, but not metalloproteases.

品質等級

形狀

lyophilized

製造商/商標名

Calbiochem®

儲存條件

OK to freeze
desiccated (hygroscopic)

運輸包裝

wet ice

儲存溫度

−20°C

相關類別

一般說明

四种蛋白酶抑制剂的混合物,用于抑制丝氨酸、半胱氨酸,但不用于抑制金属蛋白酶。推荐用于哺乳动物细胞裂解液和组织提取物品。以单瓶或10瓶一组的形式提供。复溶时,每个小瓶含有100X的贮备溶液。当稀释至1X时,请混合以下成分:
四种蛋白酶抑制剂的混合物,用于抑制丝氨酸、半胱氨酸,但不用于抑制金属蛋白酶。用1 ml H2O复溶每个小瓶,以获得100X溶液。1X贮备溶液,含有500 µM AEBSF、HCl(目录号101500)、150 nM抑肽酶(目录号616370)、1 µM E-64(目录号324890)和1 µM亮抑蛋白酶肽(目录号108975)。随附数据表。

特異性

抑制丝氨酸蛋白酶、半胱氨酸蛋白酶。切勿抑制金属蛋白酶。

生化/生理作用

主靶
丝氨酸和半胱氨酸蛋白酶,但不用于抑制金属蛋白酶。
产物不与ATP竞争。
可逆:否
细胞可渗透性:否

警告

毒性:标准处理(A)

重構

复溶后,等分并冷冻保存(-20°C)。贮备溶液在-20°C下可稳定保存至多6个月。
用1 ml H₂O复溶每个小瓶,以获得100X溶液。

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

象形圖

Corrosion

訊號詞

Danger

危險聲明

危險分類

Eye Dam. 1 - Skin Corr. 1A

儲存類別代碼

8A - Combustible corrosive hazardous materials

水污染物質分類(WGK)

WGK 3


分析證明 (COA)

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Mario Lebendiker et al.
Methods in molecular biology (Clifton, N.J.), 1485, 257-273 (2016-10-13)
Maltose-Binding Protein (MBP) is one of the most popular fusion partners being used for producing recombinant proteins in bacterial cells. MBP allows the use of a simple capture affinity step on Amylose-Agarose or Dextrin-Sepharose columns, resulting in a protein that
Emery T Usher et al.
The Journal of biological chemistry, 296, 100693-100693 (2021-04-25)
Speckle-type POZ protein (SPOP) is a ubiquitin ligase adaptor that binds substrate proteins and facilitates their proteasomal degradation. Most SPOP substrates present multiple SPOP-binding (SB) motifs and undergo liquid-liquid phase separation with SPOP. Pancreatic and duodenal homeobox 1 (Pdx1), an
Kirby Martinez-Fonts et al.
Nature communications, 11(1), 477-477 (2020-01-26)
Proteins are targeted to the proteasome by the attachment of ubiquitin chains, which are markedly varied in structure. Three proteasome subunits-Rpn10, Rpn13, and Rpn1-can recognize ubiquitin chains. Here we report that proteins with single chains of K48-linked ubiquitin are targeted
Jin Wen et al.
Bioorganic & medicinal chemistry, 28(20), 115711-115711 (2020-10-18)
Cyclic peptides are capable of binding to challenging targets (e.g., proteins involved in protein-protein interactions) with high affinity and specificity, but generally cannot gain access to intracellular targets because of poor membrane permeability. In this work, we discovered a conformationally
Chinyere Agbaegbu Iweka et al.
Journal of neurochemistry, 157(3), 494-507 (2020-12-16)
Phospholipid Phosphatase-Related Protein Type 1 (PLPPR1) is a member of a family of lipid phosphatase related proteins, integral membrane proteins characterized by six transmembrane domains. This family of proteins is enriched in the brain and recent data indicate potential pleiotropic

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