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重要文件

L9795

Sigma-Aldrich

乳酸氧化酶 来源于绿色气球菌

lyophilized powder

同義詞:

L-乳酸单加氧酶, 乳酸加氧酶, 乳酸单加氧酶, 乳酸氧化脱羧酶, 乳酸氧化酶

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About This Item

分類程式碼代碼:
12352204
NACRES:
NA.54
暫時無法取得訂價和供貨情況

生物源

bacterial (Aerococcus viridans)

品質等級

形狀

lyophilized powder

分子量

80 kDa (gel filtration)

儲存溫度

−20°C

一般說明

乳酸氧化酶(LOX)是一种黄素单核苷酸(FMN)依赖型酶,是α-羟酸氧化酶黄素酶(flavoenzyme)家族一员。

應用

来自绿色气球菌 的乳酸氧化酶已被用于:
  • 作为培养基增补剂用于将胞外乳酸转化为丙酮酸
  • 涂布在玻璃珠表面用于以推拉式喷头系统(push-pull nozzle system)进行单个细胞的乳酸电化学检测
  • 涂布在多枝化金纳米颗粒(GNP)表面用于以纸基多路复用设备(multiplexed device)同时比色测定唾液中的乳酸

生化/生理作用

在FMN辅助因子存在的情况下,乳酸氧化酶(LOX)可催化乳酸氧化形成丙酮酸和过氧化氢。该酶通过测定反应中形成的过氧化氢,有助于间接测定L-乳酸。

單位定義

一个单位定义为在标准测定条件下,在 37℃ 下,每分钟产生 1 mmol 过氧化氢的酶量。

象形圖

Health hazardExclamation mark

訊號詞

Danger

危險分類

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


從最近期的版本中選擇一個:

分析證明 (COA)

Lot/Batch Number

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Nicholas X Williams et al.
Nature electronics, 4(4), 261-268 (2022-04-05)
Electronic waste can lead to the accumulation of environmentally and biologically toxic materials and is a growing global concern. Developments in transient electronics-in which devices are designed to disintegrate after use-have focused on increasing the biocompatibility, whereas efforts to develop
Ingar Leiros et al.
Acta crystallographica. Section F, Structural biology and crystallization communications, 62(Pt 12), 1185-1190 (2006-12-05)
The crystal structure of L-lactate oxidase (LOX) from Aerococcus viridans has been determined at 2.1 A resolution. LOX catalyzes the flavin mononucleotide (FMN) dependent oxidation of lactate to pyruvate and hydrogen peroxide. LOX belongs to the alpha-hydroxy-acid oxidase flavoenzyme family;
Lin Zhou et al.
Analytical chemistry, 93(24), 8680-8686 (2021-06-10)
Metabolism of a single cell, even within the same organization, differs from other cells by orders of magnitude. Single-cell analysis provides key information for early diagnosis of cancer as well as drug screening. Any slight change in the microenvironment may
Yashwanth Ashok et al.
PloS one, 15(2), e0223870-e0223870 (2020-02-25)
Lactate oxidases belong to a group of FMN-dependent enzymes and they catalyze a conversion of lactate to pyruvate with a release of hydrogen peroxide. Hydrogen peroxide is also utilized as a read out in biosensors to quantitate lactate levels in
Cyrielle L Bouchez et al.
STAR protocols, 3(2), 101403-101403 (2022-05-24)
Lactate is a central metabolite in energy metabolism and is also involved in cell signaling and epigenetic regulations. Here, we describe an NADH-independent enzymatic assay allowing rapid, selective, and sensitive quantification of L-lactate down to the pmol range. We detail

文章

Professor Rivnay (Northwestern University, USA) discusses using organic mixed conductors as an alternative to efficiently bridge the ionic world of biology with contemporary microelectronics.

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