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G8540

Sigma-Aldrich

L-谷氨酰胺

99.0-101.0%, suitable for cell culture, non-animal source, meets USP testing specifications

同義詞:

(S)-2,5-二氨基-5-氧代戊酸, L-谷氨酸-5-酰胺, 左谷酰胺

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About This Item

線性公式:
H2NCOCH2CH2CH(NH2)CO2H
CAS號碼:
分子量::
146.14
Beilstein:
1723797
EC號碼:
MDL號碼:
分類程式碼代碼:
12352209
eCl@ss:
32160406
PubChem物質ID:
NACRES:
NA.26


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產品名稱

L-谷氨酰胺, meets USP testing specifications, suitable for cell culture, 99.0-101.0%, from non-animal source

生物源

non-animal source

品質等級

agency

meets USP testing specifications

化驗

99.0-101.0%

形狀

powder

光學活性

[α]20/D 31.5 to 33.0°, c = 10 in 2 M HCl
[α]20/D 6.3 to 7.3°, c = 4 in water

技術

cell culture | mammalian: suitable

雜質

endotoxin, tested

顏色

white

mp

185 °C (dec.) (lit.)

溶解度

H2O: 25 mg/mL

正離子痕跡

As: ≤1 ppm
Fe: ≤10 ppm
NH4+: ≤0.10%

應用

agriculture

官能基

amide

儲存溫度

room temp

SMILES 字串

N[C@@H](CCC(N)=O)C(O)=O

InChI

1S/C5H10N2O3/c6-3(5(9)10)1-2-4(7)8/h3H,1-2,6H2,(H2,7,8)(H,9,10)/t3-/m0/s1

InChI 密鑰

ZDXPYRJPNDTMRX-VKHMYHEASA-N

基因資訊

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一般說明

L-谷氨酰胺是哺乳动物血液和组织中含量最丰富的氨基酸。它对于生化和细胞培养研究至关重要。这种成分可支持细胞生长、新陈代谢和蛋白质合成。在细胞培养过程中,它可维系细胞活力、增殖和抗体产生。在生化研究中,L-谷氨酰胺有助于代谢途径、生物分子合成和细胞过程。

應用

L-谷氨酰胺氨基酸已用于:

  • 用作无血清培养基的成分,培养小鼠耳蜗器官型细胞。 [1]
  • 作为杜氏改良Eagle′s培养基营养混合物 F-12 (DMEM/F-12) 培养基补充剂,用于培养小鼠生精小管段。[2]
  • 作为RPMI 1640 培养基的补充剂,培养人单核细胞系。[3]

生化/生理作用

L-谷氨酰胺或谷氨酰胺粉末用作合成肽、蛋白质、核苷酸、嘌呤、嘧啶、氨基糖和核酸的前体。它在促进和维持细胞功能方面发挥着重要作用。肠道、肾脏、中枢神经系统 (CNS) 神经元、肝脏和胰腺β细胞等许多细胞和组织借助高含量谷氨酰胺发挥功能。[4]谷氨酰胺可调节酸碱稳态和分解代谢信号传导。[5]谷氨酰胺在氮运输和维持细胞氧化还原状态中起着至关重要的作用。[6]在细胞培养期间,谷氨酰胺可提供细胞生长和分裂所需的氮、碳和还原当量。L-谷氨酰胺可帮助将NH4+插入生物分子。
L-谷氨酰胺是一种必需氨基酸,作为细胞培养过程中主要的能量来源而成为培养基的重要组分。L-谷氨酰胺在干粉及冷冻溶液的状态下极为稳定。然而,L-谷氨酰胺在液体培养基或储存溶液中均会相对较快的降解。最佳的细胞表现通常需要在使用前向培养基中添加L-谷氨酰胺补充剂。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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分析證明 (COA)

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存取文件庫

Glutamine and glutamate?their central role in cell metabolism and function
Newsholme P, et al.
Cell Biochemistry and Function, 21(1) (2003)
Curcumin Alters Neural Plasticity and Viability of Intact Hippocampal Circuits and Attenuates Behavioral Despair and COX-2 Expression in Chronically Stressed Rats
Ga-Young Choi
Mediators of Inflammation (2017)
Interleukin-6 and IL-6 receptor cell expression in testis of rats with autoimmune orchitis
Rival C, et al.
Journal of Reproductive Immunology, 70(1-2), 43-58 (2006)
Alina Sadaf et al.
Experimental biology and medicine (Maywood, N.J.), 245(2), 146-154 (2020-01-28)
Oxidative stress is an important contributor to the pathophysiology of sickle cell disease. The pathways involved are complex and interlinked. L-glutamine is an amino acid with myriad roles in the body, including the synthesis of antioxidants, such as reduced glutathione
Lehninger Principles of Biochemistry Edition 4th null

文章

Importance and uses of glutamine in hybridoma and mammalian cell culture

谷氨酰胺在雜交瘤和哺乳動物細胞培養中的重要性和用途

Amino Acid Codon Wheel for fast RNA translation. Find which amino acid is translated from your RNA sequence quickly and easily.

Amino Acid Codon Wheel 用於快速 RNA 翻譯。從您的 RNA 序列快速輕鬆地找出哪個氨基酸被翻譯出來。

Chromatograms

application for HPLCapplication for HPLC

Questions

1–6 of 6 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  3. Hello, is the weight of G8540-10MG 10mg or 10g?

    1 answer
    1. The product SKU G8540-10MG is 10 milligram package.

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  4. How long it stable in media stored in 4℃?

    1 answer
    1. TS Final Answer: Classical media products containing 2 mM L-glutamine are stored at 2-8°C. These prepared solutions are assigned a shelf life of approximately 18 months. At similar or higher concentrations, in a pH range of 7.2 - 7,6, when protected from light, a similar shelf life is expected. Please see the image below to review the published stability study of L-Glutamine.

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  5. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  6. Is the molecular weight of L-Glutamine 146.14 or 146.15? The label information changed.

    1 answer
    1. Based on a molecular formula of C5H10N2O3, the molecular weight to seven significant figures is 146.1456. Over the years, this value may have been reported in Sigma-Aldrich documentation or on labels as either 146.15 (by rounding up) or 146.14 (by truncation). The material itself has not changed.

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