L3888

D -莱氏乳杆菌乳酸脱氢酶

lyophilized powder, 150-500 units/mg protein

• 同義詞: (R)-乳酸:NAD⁺ 氧化还原酶, D-LDH
• CAS號碼: 9028-36-8
• 酶委員會編號: 1.1.1.28 (BRENDA, IUBMB)
• EC號碼: 232-829-0
• MDL號碼: MFCD00130907
• 分類程式碼代碼: 12352204
• NACRES: NA.54

屬性

• 生物源: bacterial (Lactobacillus leichmannii)
• 品質等級: 200
• 形狀: lyophilized powder
• 比活性: 150-500 units/mg protein
• 成份: Protein, ~50% biuret
• 異物活動: Malic dehydrogenase <0.5% of base activity
• 儲存溫度: −20°C

描述

一般說明

Research area: Cell Signaling
Lactate Dehydrogenase (LDH) is classified as an oxidoreductase and is found in various organisms, including both plants and animals. LDH is widely distributed across all tissues, with high concentrations in muscle, kidney, and liver. The genes encoding LDH are LDHA, LDHB, LDHC, and LDHD. The D-isomer is produced by LDHD. There are two types of D-LDHs: NAD-dependent D-LDHs and FAD-dependent D-LDHs.

應用

在食品工业，主要催化作用是将NADH和H⁺转化成NAD⁺ ，心肌黄酶将无荧光的刃天青转化成强荧光物质试卤灵，以测量食品中D-乳酸的含量。

D-Lactic Dehydrogenase from Lactobacillus leichmannii has been used:

• in lactate dehydrogenase activity for testing the chaperone activity of proteins
• to test the kinase activities of purified thiamine monophosphate(ThiM)
• in NADH-coupled steady-state ATPase assay
• to determine cellular lactate

生化／生理作用

It acts as a crucial checkpoint in gluconeogenesis and DNA metabolism. Elevated levels of LDH in the blood have been observed in various conditions, including heart attacks, cancers, liver disease, muscle trauma, anemia, bone fractures, and infections such as encephalitis, human immunodeficiency virus(HIV), and meningitis. LDH also serves as a non-specific marker of tissue turnover, which is a normal metabolic process. Additionally, reduced D-LDH activity has been found in case of mutations in LDHD found in patients with D-lactic acidosis.

D-乳酸脱氢酶催化丙酮酸转化成 D-乳酸，同时将NADH氧化成NAD⁺。D-乳酸脱氢酶还可以催化逆反应，将D-乳酸转化成丙酮酸，同时将NAD⁺还原成NADH。

單位定義

D-乳酸脱氢酶催化丙酮酸转化成 D-乳酸，同时将NADH氧化成NAD⁺。 D-乳酸脱氢酶还可以催化逆反应，将 D-乳酸转化成丙酮酸，同时将NAD⁺ 还原成NADH。

在pH 7.0、25℃条件下，一单位酶每分钟可将1.0 μmM丙酮酸还原成D-乳酸。

外觀

含有磷酸缓冲盐的冻干粉

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable
• 個人防護裝備: Eyeshields, Gloves, type N95 (US)