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Merck
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重要文件

G7654

Sigma-Aldrich

凝胶上样液

for NA electrophoresis, solution

同義詞:

DNA Gel Loading Solution, Gel Loading Buffer

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About This Item

分類程式碼代碼:
12161703
NACRES:
NA.25
暫時無法取得訂價和供貨情況

一般說明

凝胶上样溶液在电泳期间可用作跟踪染料。染料具有轻微的负电荷,并且与DNA的迁移方向相同,从而使得用户可以监测分子在凝胶中移动的进程。迁移速率随凝胶组成而变化。上样前用样品进行1:6稀释。

應用

适用于琼脂糖或非变性聚丙烯酰胺凝胶电泳(PAGE),该电泳步骤可能是Northern和Southern印迹杂交过程的一部分。

成分

凝胶上样缓冲液含有0.25%溴酚蓝、0.25%二甲苯蓝和40%蔗糖。

其他說明

预期条带迁移如下:
在聚丙烯酰胺凝胶上,二甲苯氰基可与大约450-460bp的DNA共迁移,而溴酚蓝与15-100bp的DNA共迁移。在0.5-1.4%琼脂糖凝胶上,二甲苯氰基可与4kb dsDNA共迁移,而溴酚蓝则与300bp dsDNA共迁移。

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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S Henry et al.
Vox sanguinis, 70(1), 21-25 (1996-01-01)
While screening Le(a+b+)Polynesian DNA samples for a candidate Se(w) allele, a point mutation (C571-->T) resulting in a new stop codon (Arg191-->stop) in the alpha(1,2)fucosyltransferase gene (FUT2) was identified. This point mutation resulted in the gaining of a new restriction enzyme
David R Macinga et al.
Antimicrobial agents and chemotherapy, 47(8), 2526-2537 (2003-07-25)
We have characterized an early series of 5,6-bridged dioxinoquinolones which behaved strikingly different from typical quinolones. The 5,6-bridged dioxinoquinolones inhibited Escherichia coli DNA gyrase supercoiling activity but, unlike typical quinolones, failed to stimulate gyrase-dependent cleavable complex formation. Analogous unsubstituted compounds
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 6-6 (1989)
Peter Konings et al.
Nature protocols, 7(2), 281-310 (2012-01-21)
We present a protocol for reliably detecting DNA copy number aberrations in a single human cell. Multiple displacement-amplified DNAs of a cell are hybridized to a 3,000-bacterial artificial chromosome (BAC) array and to an Affymetrix 250,000 (250K)-SNP array. Subsequent copy
Jung-Lim Lee et al.
Journal of microbiological methods, 67(3), 456-462 (2006-12-22)
Ethidium bromide monoazide (EMA) was utilized to selectively allow conventional PCR amplification of target DNA from viable but not dead cells from a broth culture of bacterial mixed flora derived from cod fillets. The universal primers designated DG74 and RW01

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