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一般說明
凝胶上样溶液在电泳期间可用作跟踪染料。染料具有轻微的负电荷,并且与DNA的迁移方向相同,从而使得用户可以监测分子在凝胶中移动的进程。迁移速率随凝胶组成而变化。上样前用样品进行1:6稀释。
應用
适用于琼脂糖或非变性聚丙烯酰胺凝胶电泳(PAGE),该电泳步骤可能是Northern和Southern印迹杂交过程的一部分。
成分
凝胶上样缓冲液含有0.25%溴酚蓝、0.25%二甲苯蓝和40%蔗糖。
其他說明
预期条带迁移如下:
在聚丙烯酰胺凝胶上,二甲苯氰基可与大约450-460bp的DNA共迁移,而溴酚蓝与15-100bp的DNA共迁移。在0.5-1.4%琼脂糖凝胶上,二甲苯氰基可与4kb dsDNA共迁移,而溴酚蓝则与300bp dsDNA共迁移。
在聚丙烯酰胺凝胶上,二甲苯氰基可与大约450-460bp的DNA共迁移,而溴酚蓝与15-100bp的DNA共迁移。在0.5-1.4%琼脂糖凝胶上,二甲苯氰基可与4kb dsDNA共迁移,而溴酚蓝则与300bp dsDNA共迁移。
相關產品
產品號碼
描述
訂價
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類(WGK)
WGK 2
閃點(°F)
Not applicable
閃點(°C)
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。
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