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F2637

Sigma-Aldrich

Ficoll® 400

BioXtra, for molecular biology, lyophilized powder

同義詞:

多聚蔗糖 400

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About This Item

CAS號碼:
MDL號碼:
分類程式碼代碼:
12352200
NACRES:
NA.52
暫時無法取得訂價和供貨情況

生物源

non-animal source

等級

Molecular Biology
for molecular biology

產品線

BioXtra

形狀

lyophilized powder

異物活動

DNase (Exonuclease), NICKase (Endonuclease), RNase and Protease, none detected

儲存溫度

room temp

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一般說明

Ficoll® 400是蔗糖的非离子合成聚合物,可用于细胞分离和器官分离。

應用

用于细胞分离和细胞器分离。
适用于
  • 杂交实验,以减少标记探针与膜的非特异性结合[1]
  • 制备DNA上样缓冲液(15% Ficoll®,0.25%溴酚蓝和0.25%二甲苯蓝FF)
  • 分离密度相似但大小不同的细胞(来自人外周血、骨髓和脐带血的PBMC)
  • 产生密度梯度,以便分离亚细胞成分
  • 细胞和组织样品的玻璃化。[2]
  • 比较不同灌注液对缺氧性肺血管收缩(HPV)的影响。[3]

特點和優勢

  • 不含DNA酶、RNA酶、磷酸酶和蛋白酶
  • 可保留分离细胞的功能和形态完整性

其他說明

一种非离子型合成蔗糖聚合物。

法律資訊

Ficoll is a registered trademark of Cytiva

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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存取文件庫

Hae Young Yoo et al.
Pulmonary circulation, 3(2), 396-405 (2013-09-10)
Hypoxic pulmonary vasoconstriction (HPV) is a compensatory physiological mechanism in the lung that optimizes the matching of ventilation to perfusion and thereby maximizes gas exchange. Historically, HPV has been primarily studied in isolated perfused/ventilated lungs; however, the results of these
Ivana Gessara et al.
Stem cell reports, 16(4), 784-796 (2021-03-20)
The ability to genetically manipulate organisms has led to significant insights into functional genomics in many species. In birds, manipulation of the genome is hindered by the inaccessibility of the one-cell embryo. During embryonic development, avian primordial germ cells (PGCs)
Gaye Cetinkaya et al.
Cryobiology, 63(3), 292-297 (2011-10-25)
Preservation of cell and tissue samples from endangered species is a part of biodiversity conservation strategy. Therefore, setting up proper cell and tissue cryopreservation methods is very important as these tissue samples and cells could be used to reintroduce the
Cristina Batlle et al.
Cell reports, 30(4), 1117-1128 (2020-01-30)
Prion-like proteins form multivalent assemblies and phase separate into membraneless organelles. Heterogeneous ribonucleoprotein D-like (hnRNPDL) is a RNA-processing prion-like protein with three alternative splicing (AS) isoforms, which lack none, one, or both of its two disordered domains. It has been
Zhaoming Yang et al.
Frontiers in endocrinology, 11, 587450-587450 (2020-12-29)
Islet culture prior to transplantation is a standard practice in many transplantation centers. Nevertheless, the abundant islet mass loss and function impairment during this serum-deprivation culture period restrain the success of islet transplantation. In the present study, we used a

文章

Centrifugation enables the separation of particles by sedimentation. Learn how to separate particles using a centrifuge and how to use Stokes' law to calculate the velocity of sedimentation.

離心法可藉由沉降將微粒分離。學習如何使用離心機分離微粒,以及如何使用斯托克斯定律計算沉降速度。

條款

Ficoll 400® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.

Ficoll 400® 是一種非離子聚合物,具有高親水性和水溶性,適合各種生物應用。

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

學習 Northern 和 Southern 印迹的基本知識,以及將大分子轉移到膜支持物上的協議和應用。

Questions

1–6 of 6 Questions  
  1. Do you have a protocols for use of Product F2637, Ficoll, in various applications?

    1 answer
    1. Please check the product information sheet for F4375 which has a detailed protocol.

      Helpful?

  2. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  3. Can Product F2637, Ficoll, be used as a component of a DNA loading buffer?

    1 answer
    1. This product can be used in a DNA loading buffer with the following recipe to prepare a 6X stock:0.25% bromophenol blue0.25% xylene cyanol FF15% Ficoll

      Helpful?

  4. What is the difference between Ficoll 400 and Ficoll PM 400?

    1 answer
    1. The difference between Ficoll 400 and Ficoll PM 400 is the PM 400 has stricter specifications on the number of microorganisms/g and the endotoxin level is < 0.125 EU/ml

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  5. Can you use Product F2637, Ficoll, to isolate mononuclear cells?

    1 answer
    1. When trying to isolate mononuclear cells you can use this product but we offer Histopaque that is a mixture of Ficoll and Hypaque. For humans, we offer H8889, Histopaque®-1077 Hybri-Max™ or 1077-1, Histopaque®-1077. For rodents we offer 1083-1, Histopaque®-1083. For isolation of neutrophils, we offer 1119-1, Histopaque®-1119.

      Helpful?

  6. Is Product F2637, Ficoll, the same as Ficoll 400?

    1 answer
    1. Yes. It is Ficoll 400.  This product is graded molecular biology grade and is free of any nucleases

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