F1757

Fumarase from porcine heart

ammonium sulfate suspension, ≥300 units/mg protein (biuret)

• 同義詞: L-Malate hydrolyase, Fumarate hydratase
• CAS號碼: 9032-88-6
• 酶委員會編號: 4.2.1.2 (BRENDA, IUBMB)
• EC號碼: 232-887-7
• MDL號碼: MFCD00131111
• 分類程式碼代碼: 12352204
• NACRES: NA.54

屬性

• 形狀: ammonium sulfate suspension
• 比活性: ≥300 units/mg protein (biuret)
• 異物活動: malic dehydrogenase ≤0.1%
• 儲存溫度: 2-8°C

描述

一般說明

Fumarase (FH in human) is a well-known tricarboxylic-acid-cycle enzyme found in both the cytoplasm and mitochondria of all eukaryotes.[1]

Fumarase is mapped to human chromosome 1q43.[2] Fumarase comprises of three domains D1, D2 and D3 and belongs to class II fumarase family.[3]

應用

Fumarase from porcine heart has been used:

• as a positive control in isocitrate dehydrogenase and α-ketoglutarate dehydrogenase assay[4]
• for immobilized enzyme based stability assay[5]
• in fumarate hydratase assays in human lung carcinoma cells (A549)[6]

Fumarase is used as a protein calibration standard in the purification of intact DNA polymerase a/primase from mouse cells.[7]

生化／生理作用

Fumarase catalyzes the reversible hydration of fumarate to malate. In its mitochondrial form, fumarate is involved in the Krebs Cycle, while the cytosolic form is involved in amino acid metabolism.

Fumarase is essential for regulation of cellular metabolism.[8] Fumarase elicits protective function from double-strand breaks (DSBs) induced by infrared radiation.[9] Loss of function mutations in the fumarase gene is implicated in hereditary leiomyomatosis and renal cell cancer (HLRCC)-syndrome.[2][10]

單位定義

One unit will convert 1.0 μmole of L-malate to fumarate per min at pH 7.6 at 25 °C.

外觀

Suspension in 3.2 M (NH₄)₂SO₄, 0.05 M KH₂PO₄, pH 7.5, 0.014 M 2-mercaptoethanol

準備報告

Isolated by the method of Beeckmans, S., and Kanarek, L., Eur. J. Biochem., 78, 437 (1977). Affinity purified.

安全資訊

• 象形圖: GHS07
• 訊號詞: Warning
• 危險聲明: H317
• 防範說明: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• 危險分類: Skin Sens. 1
• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable