Fumarase (or fumarate hydratase) is an enzyme that catalyzes the reversible hydration/dehydration reaction of fumarate to malate. Fumarase exists in two isoforms: a cytosolic and mitochondrial form. In the citric acid cycle, it facilitates a transition step in the production of energy in the form of NADH. Fumarase deficiency in humans results in early brain development problems and is characterized by poor feeding, hypotonia, failure to thrive, etc.
特點和優勢
Linear detection range: 0.4 to 70 U/L
Compatible with high-throughput handling systems.
適合性
The kit is suitable for fumarase activity determination in biological samples (e.g. plasma, serum, erythrocytes, tissue and culture media.)
原則
The Fumarase Assay Kit is a non-radioactive, colorimetric assay based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is proportional to the enzyme activity.
