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H2500

Sigma-Aldrich

血红蛋白 来源于牛血液

lyophilized powder

同義詞:

Hb, 牛血红蛋白, 高铁血红蛋白

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About This Item

CAS號碼:
MDL號碼:
分類程式碼代碼:
12352202
eCl@ss:
42030116
NACRES:
NA.61
暫時無法取得訂價和供貨情況

生物源

bovine blood

形狀

lyophilized powder

分子量

Mr ~64500

技術

MALDI-TOF: suitable

儲存溫度

2-8°C

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一般說明

血红蛋白是红细胞的主要成分,是其具有红色颜色的原因。它在红细胞中的正常浓度为34%。[1]血红蛋白是一种带有α和β链的球状蛋白,其分别含有141和146个氨基酸。它以四聚体的形式存在,每个单体都带有由铁元素所构成的血红素的杂环卟啉环。[1]

應用

α-弹性蛋白的溶解度已被应用于弹性蛋白模拟生物材料的构建。
牛血中的血红蛋白已被用于:
  • 在脊髓匀浆中利用Darbkin′检测、四极离子淌度飞行时间质谱用于定量脑实质性内出血[2]和实质性出血[3]的标准曲线生成[4]
  • 在结晶研究中生成分子印迹聚合物(MIP)以模拟高分子量聚乙二醇(PEG)[5]

生化/生理作用

氧气转运蛋白。Fe2+/Fe3+平衡是血液氧合的一种生理指标;脱氧血红蛋白通过将亚硝酸还原为NO来补充反馈回路,而NO是一种可增强血液流向缺氧组织流量的血管扩张剂。
氧转运体,NO 清除剂
由于血红蛋白能够将氧从肺部输送到身体组织,并促进二氧化碳的返回运输,因此它是脊椎动物最重要的呼吸蛋白。[1]牛血红蛋白可用于生成血红蛋白囊泡(HbV)以进行氧气运输。[6]与人血红蛋白相比,它具有热稳定性并对氧气显示出了高亲和性。[6]牛血红蛋白可与合成染料以及偶氮染料相互作用。[7]推荐使用聚合形式的牛血红蛋白治疗自身免疫性溶血性贫血。[8]

注意

由于天然血红蛋白在空气中易于氧化,因此这些制剂主要为高铁血红蛋白。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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Questions

1–2 of 2 Questions  
  1. Does the website specify if H2500 Hemoglobin from bovine blood is ferrohemoglobin or oxyhemoglobin?

    1 answer
    1. The website indicates that the product H2500 is mainly methemoglobin. A search of the website did not uncover any products marketed as either ferrohemoglobin or oxyhemoglobin.

      Helpful?

  2. What is the difference between H2500 and H2625 when making Chocolate agar with bovine hemoglobin? Although they share the same CAS and Specification Sheet, there is a significant difference in price between the two.

    1 answer
    1. Products H2500 and H2625 are not suitability tested for use in producing Chocolate Agar. The key differences between H2500 and H2625 lie in their purity and intended uses. H2500 is a purer product, suitable for MALDI-TOF, and associated with a companion product for HPLC analysis of hemoglobin variants. It undergoes two steps of ammonium sulfate precipitation, filtration, dialysis, and lyophilization. On the other hand, H2625 is not suitability tested for use as a protease substrate and lacks the ammonium sulfate precipitation step. These distinctions likely contribute to the significant price difference between the two products.

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