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重要文件

MAK193

Sigma-Aldrich

柠檬酸合成酶活性检测试剂盒

Citrate Synthase Activity Assay Kit

Sufficient for 100 Colorimetric tests

同義詞:

柠檬酸合成酶检测试剂盒

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About This Item

EC號碼:
分類程式碼代碼:
12161503
NACRES:
NA.84
暫時無法取得訂價和供貨情況

檢測方法

colorimetric

相關疾病

endocrinological disorders, diabetes; cancer

儲存溫度

−20°C

基因資訊

human ... CS(1431)
mouse ... CS(12974)
rat ... CS(170587)

一般說明

柠檬酸合酶(CS;EC 2.3.3.1)是一种线粒体酶,存在于所有能够进行氧化代谢的细胞中。它在Krebs 三羧酸循环中,催化草酰乙酸合成柠檬酸盐。1,2柠檬酸合酶参与能量产生、脂肪生成和胆固醇生成,其活性遵循昼夜节律模式。[1]

應用

柠檬酸合酶活性检测试剂盒已用于测量样品中柠檬酸合酶的活性。[2]

適合性

适于测定不同生物样品内的柠檬酸合成酶活性,如细胞和组织培养上清液以及纯化所得的线粒体。

原則

柠檬酸合酶活性检测试剂盒提供了一种简单直接的方法,用于测量各种组织、细胞培养物(粘附或悬浮)和纯化线粒体中柠檬酸合酶的活性。使用偶联酶反应测定柠檬酸合酶活性,其产生与存在的酶活性成比例的比色(412 nm)产物。一个单位的柠檬酸合酶是在25 °C和pH 7.2 下,每分钟产生 1.0 mmole CoA的酶的量。

僅套裝組件

產品號碼
描述

  • CS Assay Buffer

  • CS Substrate Mix

  • CS Developer

  • GSH Standard, Reduced

  • CS Positive Control

象形圖

Health hazard

訊號詞

Danger

危險聲明

危險分類

Eye Irrit. 2 - Resp. Sens. 1

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3


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分析證明 (COA)

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Questions

  1. Is the sensitivity information for the CS0720 (Citrate Synthase Assay Kit) available?

    1 answer
    1. The lower concentration tested for the product CS0720 (Citrate Synthase Assay Kit) is 1u/µgP

      Helpful?

Reviews

Active Filters

  1. USA
    • Review 1
    • Votes 0
    1 out of 5 stars.

    This actually measures total deacetylation activity, not specific to CS

    The mechanism for the assay kit is the deacetylation of Acetyl-CoA to CoA. There are multiple enzymes can do this, not just CS. This assay kit doesn't have a good control for the background non-CS-related deacetylation step, so it is not the right product to measure CS activity. CS0720 is the CS specific assay kit to buy.

    Helpful?

    1. Response from MilliporeSigma:

      MAK193 has been discontinued, CS0720 is its recommended replacement. CS0720 includes a positive control (which MAK193 lacked). CS0720 provides the Acetyl Coenzyme A and the Oxaloacetic acid needed for Citrate Synthase to produce Citrate.

      We encourage customers who experience problems with our products to contact us for additional technical support. Please visit https://www.sigmaaldrich.com/US/en/support/customer-support to submit a Product Technical Inquiry.

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