Sigma 公司的 DirectLoad ™ 50 bp DNA Step Ladder 包含 17 个平端片段，由 50～500 bp 的 50 bp 重复序列、600～900 bp 的 100 bp 重复序列和 1～3kb 的 1kb 重复序列组成。可以在琼脂糖或聚丙烯酰胺凝胶上的单一泳道中直接上样 10ul 的梯子。
應用
DirectLoad™ 50 bp DNA Step Ladder 已用于细胞裂解和基因组剪切检测分析。[1]
适于通过琼脂糖或聚丙烯酰胺凝胶测定PCR产生的DNA片段的大小。
特點和優勢
可直接上样
易于使用
常见的条带大小
成分
5% 甘油、4.2 mM EDTA、0.083% 橙黄 G 和 0.0125% 二甲苯蓝的凝胶上样溶液中提供了 Sigma 的 DirectLoad ™ 50 bp DNA 梯形条带。
其他說明
为获得最佳分离度，推荐琼脂糖凝胶浓度为 2.0%。
法律資訊
DirectLoad is a trademark of Sigma-Aldrich Co. LLC
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Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.
Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.
