This DNA ladder can be used with a loading buffer such as G2526:
https://www.sigmaaldrich.com/product/sigma/g2526
biological source
synthetic
Quality Segment
grade
Molecular Biology
form
liquid
usage
75 uses
suitability
suitable for electrophoresis (DNA)
storage temp.
−20°C
General description
PCR 100 bp low ladder 专为测定聚合酶链反应(PCR)产生的DNA片段的大小而设计。
Sigma 的 PCR 100 bp 低标记包含来自 100-1,000 个碱基的 10 个 dsDNA 片段,均匀分布在 100 bp 的重复序列中。2ul 的梯状物应在凝胶上样缓冲液中稀释,然后上样到琼脂糖凝胶上的单一泳道中。
Application
PCR 100 bp Low Ladder已用于:
- 在琼脂糖凝胶电泳中作为标准DNA标记,以获得约240 bp的DNA片段,用于聚合酶链反应(PCR)-变性梯度凝胶电泳(DGGE)分析[1]
- 从酶消化PCR混合物中可视化DNA
- 估计PCR产物片段的分子量
Other Notes
10 mM Tris-HCl (pH 8.0) 和 1.0 mM EDTA 溶液中提供 Sigma 的 PCR 100 bp 低标记物。
为获得最佳分离度,推荐琼脂糖凝胶浓度为 2.0%。
包含 10 个条带,100-1,000bp,精确的 100bp 间隔(梯状)重组重复序列。
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本產品 | |||
|---|---|---|---|
| biological source synthetic | biological source - | biological source - | biological source fermentation/recombinant (E. coli strain DH5A) |
| grade Molecular Biology | grade Molecular Biology | grade - | grade Molecular Biology |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
| form liquid | form liquid | form liquid | form liquid |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| suitability suitable for electrophoresis (DNA) | suitability suitable for electrophoresis (DNA) | suitability suitable for electrophoresis (DNA) | suitability suitable for electrophoresis (DNA) |
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What loading buffer/dye is the P1473 DNA ladder compatible with for loading onto a gel.
1 answer-
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