The concentration of the DNA marker is not part of the specification and that information is not available. Product DPLUS-1K - DirectLoad™ Plus 1kb DNA Ladder is a comparable marker that may be considered an alternative. DPLUS-1KT is noted as having a DNA concentration of 100 ug/ul. The DNA concentration for individual bands is unavailable.
D7058
DirectLoad™ 宽分子量 DNA 标准
ready-to-use marker for DNA electrophoresis
同義詞:
DirectLoad™ marker, RTU DNA ladder, RTU DNA marker, agarose gel electrophoresis ladder, agarose gel electrophoresis marker, gel ladder, gel marker, wide range ladder, wide range ladder for gel electrophoresis, wide range marker for gel electrophoresis
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About This Item
分類程式碼代碼:
41105335
NACRES:
NA.25
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一般說明
Sigma的DirectLoad宽分子量 DNA 分子量标准包含16个从50 - 10,000 bp并带有规则间隔的片段。10 ul分子量标准可直接上样至琼脂糖或聚丙烯酰胺凝胶上的单个泳道中。
應用
适于通过琼脂糖或聚丙烯酰胺凝胶测定PCR产生的DNA片段的大小。
特點和優勢
- 可直接上样
- 易于使用
- 常见的条带大小
成分
Sigma的DirectLoad™宽分子量 DNA 分子量标准以含有2.5% Ficoll（400型）、0.0125%溴酚蓝以及0.00625%二甲苯蓝的凝胶上样溶液形式提供。
其他說明
含有16个50 - 10,000 bp片段，在琼脂糖凝胶上呈现合理间隔的条带。
为实现最佳分辨率，建议的琼脂糖凝胶浓度为0.75 - 2.0%。
法律資訊
DirectLoad is a trademark of Sigma-Aldrich Co. LLC
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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文章
使用 REDExtract-N-Amp™ PCR ReadyMix™ 和 JumpStart™ REDTaq® PCR ReadyMix™ 試劑的集落 PCR 試劑和我們的集落 PCR 方案。
Colony PCR reagents and our colony PCR protocol using REDExtract-N-Amp™ PCR ReadyMix™ and JumpStart™ REDTaq® PCR ReadyMix™ reagents.
Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.
用於凝膠電泳的標記有助於確定 DNA、PCR 片段和 RNA 的大小，與常見的核酸染色劑染色效果良好。
條款
Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures.
相關內容
Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures.
KOD One™ PCR 主混合物概述，用於高特異性、高保真度和高產量的超快速 PCR
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Could you provide the concentraion of the marker? Idealy band by band so that it can be used as an estimate of the concentration of samples run along side.
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