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重要文件

A6154

Sigma-Aldrich

抗-兔IgG(全分子)-过氧化物酶 山羊抗

affinity isolated antibody, buffered aqueous solution

同義詞:

Goat Anti-Rabbit IgG (whole molecule)–HRP

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About This Item

MDL號碼:
分類程式碼代碼:
12352203
NACRES:
NA.46
暫時無法取得訂價和供貨情況

生物源

goat

共軛

peroxidase conjugate

抗體表格

affinity isolated antibody

抗體產品種類

secondary antibodies

無性繁殖

polyclonal

形狀

buffered aqueous solution

物種活性

rabbit

技術

direct ELISA: 1:10,000

運輸包裝

dry ice

儲存溫度

−20°C

目標翻譯後修改

unmodified

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一般說明

IgG是糖蛋白抗体,可调控几种免疫应答。靶蛋白的兔IgG通常在多种研究应用中用作一抗。因此,与可检测底物缀合的抗兔IgG二抗是靶蛋白分析的有用工具。山羊抗兔IgG(全分子)-过氧化物酶抗体在缀合前通过与正常血清和兔IgG免疫电泳对兔IgG产生免疫特异性。

免疫原

纯化的兔IgG

應用

使用HRP标记的山羊抗兔IgG作为二抗,通过蛋白印迹分析C33A细胞裂解物。 对来自结肠粘膜提取物(μg)的蛋白等分试样进行蛋白印迹分析:使用HRP标记的山羊抗兔IgG作为二抗,用TBST以1:5000稀释,室温孵育1小时。
山羊抗兔IgG(全分子)-过氧化物酶抗体用于蛋白印迹(1:6,000)和ELISA检测。
用 HRP 标记的羊抗兔 IgG 作为二抗,对 C33A 细胞裂解物进行免疫共沉淀和免疫印迹分析。

外觀

0.01 M磷酸盐缓冲液(pH 7.4,含有1%牛血清白蛋白和防腐剂)溶液。

準備報告

使用Wilson, M.B.和Nakane, P.K.在Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978)中描述的高碘酸盐法制备。

免責聲明

除非我们的产品目录或产品随附的其他公司文档中另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人或动物。

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象形圖

Exclamation markEnvironment

訊號詞

Warning

危險分類

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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文章

檢視決定選擇第二抗體的關鍵因素。瞭解種類、亞類、同種型、標籤等資訊。

Review the key factors that should figure in your decision to choose a secondary antibody. Learn about species, subclass, isotype, label, and more.

Questions

  1. Per quanto tempo è possibile conservare la confezione alle condizioni specificate (flacone chiuso)?

    1 answer
    1. This product is not assigned an expiration or retest date. When stored unopened at the recommended storage temperature of -20°C, the material is quite stable. Products with no assigned expiration date are covered by a warranty of 1 year from the date of shipment. This antibody may be thawed, aliquoted, and re-frozen upon receipt. Thawed aliquots are stable for day-to-day use for up to one month at 2 - 8°C. Please see the link below for additional information provided in the product data sheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/649/246/a6154dat.pdf

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