This product is not assigned an expiration or retest date. When stored unopened at the recommended storage temperature of -20°C, the material is quite stable. Products with no assigned expiration date are covered by a warranty of 1 year from the date of shipment. This antibody may be thawed, aliquoted, and re-frozen upon receipt. Thawed aliquots are stable for day-to-day use for up to one month at 2 - 8°C. Please see the link below for additional information provided in the product data sheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/649/246/a6154dat.pdf
推薦產品
生物源
goat
共軛
peroxidase conjugate
抗體表格
affinity isolated antibody
抗體產品種類
secondary antibodies
無性繁殖
polyclonal
形狀
buffered aqueous solution
物種活性
rabbit
技術
direct ELISA: 1:10,000
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
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一般說明
IgG是糖蛋白抗体,可调控几种免疫应答。靶蛋白的兔IgG通常在多种研究应用中用作一抗。因此,与可检测底物缀合的抗兔IgG二抗是靶蛋白分析的有用工具。山羊抗兔IgG(全分子)-过氧化物酶抗体在缀合前通过与正常血清和兔IgG免疫电泳对兔IgG产生免疫特异性。
免疫原
纯化的兔IgG
應用
使用HRP标记的山羊抗兔IgG作为二抗,通过蛋白印迹分析C33A细胞裂解物。 对来自结肠粘膜提取物(μg)的蛋白等分试样进行蛋白印迹分析:使用HRP标记的山羊抗兔IgG作为二抗,用TBST以1:5000稀释,室温孵育1小时。
山羊抗兔IgG(全分子)-过氧化物酶抗体用于蛋白印迹(1:6,000)和ELISA检测。
用 HRP 标记的羊抗兔 IgG 作为二抗,对 C33A 细胞裂解物进行免疫共沉淀和免疫印迹分析。
外觀
0.01 M磷酸盐缓冲液(pH 7.4,含有1%牛血清白蛋白和防腐剂)溶液。
準備報告
使用Wilson, M.B.和Nakane, P.K.在Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978)中描述的高碘酸盐法制备。
其他說明
免責聲明
除非我们的产品目录或产品随附的其他公司文档中另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人或动物。
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訊號詞
Warning
危險分類
Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
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檢視決定選擇第二抗體的關鍵因素。瞭解種類、亞類、同種型、標籤等資訊。
Review the key factors that should figure in your decision to choose a secondary antibody. Learn about species, subclass, isotype, label, and more.
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