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A3687

Sigma-Aldrich

抗 兔 IgG(全分子)-碱性磷酸酶 山羊抗

affinity isolated antibody, buffered aqueous glycerol solution

同義詞:

Alkaline Phosphatase Secondary Antibody, Anti Rabbit Antibody - Anti-Rabbit IgG (whole molecule)–Alkaline Phosphatase antibody produced in goat, Anti Rabbit Sigma, anti rabbit antibody

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About This Item

MDL號碼:
分類程式碼代碼:
12352203
NACRES:
NA.46

生物源

goat

品質等級

共軛

alkaline phosphatase conjugate

抗體表格

affinity isolated antibody

抗體產品種類

secondary antibodies

無性繁殖

polyclonal

形狀

buffered aqueous glycerol solution

物種活性

rabbit

技術

direct ELISA: 1:30,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
western blot: 1:30,000

運輸包裝

wet ice

儲存溫度

2-8°C

目標翻譯後修改

unmodified

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一般說明

IgG是糖蛋白抗体,可调节几种免疫反应。针对靶蛋白的兔IgG通常在各种研究应用中用作初级抗体。因此,与可检测底物偶联的次级抗兔IgG是分析靶蛋白的有用工具。山羊抗兔IgG(全分子)-碱性磷酸酶抗体结合所有兔Igs。

免疫原

纯化兔 IgG 作为免疫原

應用

使用碱性磷酸酶缀合的山羊抗兔IgG作为二抗(用5%吐温/PBS按1:2000稀释),采用ELISA法分析小鼠视网膜组织中存在的低氧探针结合蛋白的水平。使用的底物是4-硝基苯基磷酸二钠盐六水合物(Sigma)。
山羊抗兔IgG(全分子)-碱性磷酸酶抗体可用于免疫印迹和ELISA。该抗体也可用于免疫组化(1:50,使用福尔马林固定、石蜡包埋切片)。
通过 ELISA 法分析 PADI4 对抗凝血酶的瓜氨酸化作用,使用碱性磷酸酶标记的羊抗兔 IgG 作为在0.05M碳酸盐/碳酸氢盐缓冲液(Ph 9.6)中以1:5000稀释的二次稀释剂。

外觀

在含有1mM MgCl 2,10mM甘氨酸,1%牛血清白蛋白,50%甘油和15mM叠氮化钠的0.05M Tris缓冲液(pH 8.0)中的溶液。

免責聲明

除非我们的产品目录或产品随附的其他公司文件中另有说明,否则我们的产品仅用于研究用途,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、体外或体内治疗用途或任何类型的人类或动物消费或应用。

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1


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