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重要文件

A2230

Sigma-Aldrich

三磷酸腺苷双磷酸酶 来源于马铃薯

High Activity, ATPase ≥600 units/mg protein, lyophilized powder

同義詞:

腺苷5′-三磷酸酶, 腺苷5′-二磷酸酶

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About This Item

CAS號碼:
酶委員會編號:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54
暫時無法取得訂價和供貨情況

生物源

potato

形狀

lyophilized powder

品質

High Activity

ATPase activity

≥600 units/mg protein

secondary activity

≥50 % of base activity ADPase

成份

protein, ≥30%

異物活動

Acid Phosphatase ≤2% of base activity

運輸包裝

wet ice

儲存溫度

−20°C

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應用

三磷酸腺苷双磷酸酶用于水解核苷三磷酸和二磷酸。 Sigma的三磷酸腺苷双磷酸酶已用于血小板聚集的抑制研究[1]。 A2230产品是一种高活性的三磷酸腺苷双磷酸酶(apyrase)。
不同种类的马铃薯(S. tuberosum)中存在至少2种同工酶:一种的ATP酶/ADP酶比例较高(∼10)和另一种较低(∼1)。
反应:ATP → ADP+Pi → AMP+2Pi

生化/生理作用

三磷酸腺苷双磷酸酶存在于所有真核生物和部分原核生物中。 马铃薯三磷酸腺苷双磷酸酶在调节生长和发育中发挥着至关重要的作用。 Apyrase可参与神经刺激后突触ATP(作为神经递质)的失活,以及抑制ADP诱导的血小板聚集,以防止血栓形成 [2]。 其活性需要二价金属离子,并且使用5mM的钙离子观察到最佳的活性。

包裝

按ATP酶单位售卖。

單位定義

在pH 6.5、30℃条件下,一单位酶每分钟可从ATP或ADP释放1.0 μmole无机磷酸。

外觀

含有琥珀酸钾缓冲盐的冻干粉

準備報告

来源于红土豆

象形圖

Health hazard

訊號詞

Danger

危險聲明

防範說明

危險分類

Resp. Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Moritz Schmelzle et al.
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Plant physiology, 160(4), 1985-1995 (2012-10-17)
Recent evidence indicates that extracellular nucleotides regulate plant growth. Exogenous ATP has been shown to block auxin transport and gravitropic growth in primary roots of Arabidopsis (Arabidopsis thaliana). Cells limit the concentration of extracellular ATP in part through the activity
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