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重要文件

A6237

Sigma-Aldrich

马铃薯来源腺苷三磷酸双磷酶

recombinant, expressed in Pichia pastoris, ATPase ≥1000 units/mg protein, lyophilized powder

同義詞:

三磷酸腺苷双磷酸酶 来源于马铃薯, 腺苷 5′-三磷酸酶, 腺苷 5′-二磷酸酶

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About This Item

酶委員會編號:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54
暫時無法取得訂價和供貨情況

重組細胞

expressed in Pichia pastoris

品質等級

形狀

lyophilized powder

ATPase activity

≥1000 units/mg protein

運輸包裝

wet ice

儲存溫度

−20°C

一般說明

腺苷三磷酸双磷酶又称 ATP-二磷酸水解酶,在各种动植物组织中都有表达。商业化的腺苷三磷酸双磷酸酶分离自马铃薯块茎。[1]

生化/生理作用

在存在二价阳离子的情况下,磷酸酯酶可水解三磷酸和二磷酸核苷的磷酸酐键。[1][2]它对核苷酸具有广泛的底物特异性。这种酶的这一特性使其适于许多不同的生物技术应用,包括 DNA 测序和血小板聚集抑制。[1]

象形圖

Health hazard

訊號詞

Danger

危險聲明

防範說明

危險分類

Resp. Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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分析證明 (COA)

Lot/Batch Number

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Xuan Zhang et al.
European journal of medicinal chemistry, 199, 112397-112397 (2020-05-11)
Targeting BCL-XL via PROTACs is a promising strategy in reducing BCL-XL inhibition associated platelet toxicity. Recently, we reported potent BCL-XL PROTAC degraders that recruit VHL or CRBN E3 ligase. However, low protein expression or mutation of the responsible E3 ligase
Subin Mao et al.
Lab on a chip, 21(16), 3128-3136 (2021-06-29)
Integrins are key players in platelet adhesion and aggregation. Integrin molecular tensions, the forces transmitted by integrin molecules, are regulated by both mechanical and biochemical cues, and the outside-in and inside-out signaling has been extensively studied. While the mechanical properties
Methylotrophic yeast Pichia pastoris as a host for production of ATP-diphosphohydrolase (apyrase) from potato tubers (Solanum tuberosum).
Nourizad N, et al.
Protein Expression and Purification, 27(2), 229-237 (2003)
Cloning, sequencing, and expression of a human brain ecto-apyrase related to both the ecto-ATPases and CD39 ecto-apyrases1.
Smith T M & Kirley T L
Biochimica et Biophysica Acta, 1386(1), 65-78 (1998)
Celia Cordero-Sanchez et al.
Blood advances, 6(15), 4471-4484 (2022-06-14)
Store-operated Ca2+-entry is a cellular mechanism that governs the replenishment of intracellular stores of Ca2+ upon depletion caused by the opening of intracellular Ca2+-channels. Gain-of-function mutations of the 2 key proteins of store-operated Ca2+-entry, STIM1 and ORAI1, are associated with

Questions

  1. Hello. Is the concentration of 100 units in 1 mL of water? If not, how can I make the dilution to obtain 100 units? Thanks for you attention! Guilherme

    1 answer
    1. The solubility of this enzyme is tested on the finished product at 1 mL per vial in water. The 100-unit package size will be readily soluble in 1 mL, however, the number of actual units per vial should be reviewed in the lot-specific certificate of analysis. The vial content is typically greater than 100 units. The reconstitution volume would have to be adjusted accordingly in order to achieve a 100 unit per milliliter concentration.
      See the link below to review the sample Certificate of analysis:
      https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/A6237-100UN0000174833.pdf

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