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重要文件

475989

Sigma-Aldrich

噻唑蓝四唑蓝

Membrane-permeable yellow dye that is reduced by mitochondrial reductases in living cells to form the dark blue product, MTT-formazan.

同義詞:

噻唑蓝四唑蓝, 3-(4,5-二甲基-2-噻唑)-2,5-二苯基-2H-溴化四唑噻唑蓝

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About This Item

經驗公式(希爾表示法):
C18H16BrN5S
CAS號碼:
分子量::
414.32
MDL號碼:
分類程式碼代碼:
12171500
NACRES:
NA.25
暫時無法取得訂價和供貨情況

品質等級

描述

RTECS - XF8060000

化驗

≥98% (silver nitrate titration)

形狀

solid

製造商/商標名

Calbiochem®

儲存條件

OK to freeze

雜質

≤0.5% Sulfated ash

顏色

yellow

溶解度

PBS: 2 mg/mL

正離子痕跡

heavy metals: ≤0.001%

運輸包裝

ambient

儲存溫度

15-25°C

SMILES 字串

[s]1c(nc(c1C)C)[n]2[n+](nc(n2)c4ccccc4)c3ccccc3.[Br-]

InChI

1S/C18H16N5S.BrH/c1-13-14(2)24-18(19-13)23-21-17(15-9-5-3-6-10-15)20-22(23)16-11-7-4-8-12-16;/h3-12H,1-2H3;1H/q+1;/p-1

InChI 密鑰

AZKSAVLVSZKNRD-UHFFFAOYSA-M

一般說明

一种膜渗透性黄色染料,在活细胞中被线粒体还原酶还原形成深蓝色产物MTT-甲臜。MTT-甲臜在540或570 nm处的分光光度测量可定量细胞活力。用作细胞毒性(如用于筛选癌症药物)和凋亡的直接指标。一种用于研究NADP依赖性脱氢酶类的电子受体。

生化/生理作用

主要靶标
用作细胞毒性和凋亡的直接指标。
产物不与ATP竞争。
可逆:否
细胞可渗透性:具有

警告

毒性:有害 & 致癌/致畸性(E)

重構

复溶后,分装并冷冻保存(-20°C)。贮备液在-20°C下可稳定保存至多1个月。

其他說明

Liu, Y., et al. 1997.J. Neurochem.69, 581.
Abdallah, M.A. 1980.Eur. J. Biochem.112, 331.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

象形圖

Health hazardExclamation mark

訊號詞

Warning

危險分類

Eye Irrit. 2 - Muta. 2 - Skin Irrit. 2 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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