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重要文件

88417

Sigma-Aldrich

MTT 甲臜

MTT Formazan

MTT reduction end product, powder

同義詞:

1-(4,5-二甲基吡啶-2-基)-3,5-二苯基甲酰胺, 噻唑基蓝甲臜

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About This Item

經驗公式(希爾表示法):
C18H17N5S
CAS號碼:
分子量::
335.43
MDL號碼:
分類程式碼代碼:
12352204
PubChem物質ID:
NACRES:
NA.83
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產品名稱

MTT 甲臜, MTT reduction end product

品質等級

形狀

powder

溶解度

chloroform: 10 mg/mL, clear, dark violet

SMILES 字串

Cc1nc(\N=N\C(=N\Nc2ccccc2)c3ccccc3)sc1C

InChI

1S/C18H17N5S/c1-13-14(2)24-18(19-13)23-22-17(15-9-5-3-6-10-15)21-20-16-11-7-4-8-12-16/h3-12,20H,1-2H3/b21-17+,23-22+

InChI 密鑰

GIFKVQOPAJDJLO-SZVLODPRSA-N

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相關類別

應用

MTT甲臜用于细胞活率检测。[1][2][3]

生化/生理作用

MTT法是一种比色反应分析方法,常用于检测细胞活率。该方法要求将溶于水的黄色3-[4,5-二甲基噻唑-2-yl]-2,5-基四氮唑嗅盐(MTT)酶促还原为不溶于水的紫色MTT-甲臜。该反应由线粒体脱氢酶催化,仅在活细胞中发生。[4][5]因此,蓝色甲臜量与活细胞数量成正比。通过分光光度法分析570 nm波长处的甲臜溶液吸光度。[6]

其他說明

评审:四唑盐和甲瓒[7]

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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Questions

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    • Review 1
    • Votes 8
    4 out of 5 stars.

    Will Not Work For MTT Proliferation Assay

    I accidentally bought this thinking it was MTT salt to use in a cell proliferation assay but it is MTT Formazan, the water insoluble end product! Therefore, it is useless for the assay. I suppose you could use it as a control to optimize your buffer for solubilizing the insoluble Formazan. The description for this product is misleading and should clarify that this is not what you need for an MTT assay, and refer the customer to MTT salt. That said, this is a nice purple crystalline powder and insoluble in water consistent with MTT Formazan, if that is what you actually want.

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