At physiological pH, phenol red strongly absorbs in the same wavelength range as the MTT assay’s readout product. Washing cells thoroughly to remove the media and phenol red minimizes this interference.
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推薦產品
產品線
BioReagent
品質等級
化驗
≥97.5% (HPLC)
形狀
powder
技術
cell culture | insect: suitable
cell culture | mammalian: suitable
mp
195 °C (dec.) (lit.)
溶解度
H2O: 5 mg/mL
儲存溫度
2-8°C
SMILES 字串
[Br-].Cc1nc(sc1C)-[n+]2nc(nn2-c3ccccc3)-c4ccccc4
InChI
1S/C18H16N5S.BrH/c1-13-14(2)24-18(19-13)23-21-17(15-9-5-3-6-10-15)20-22(23)16-11-7-4-8-12-16;/h3-12H,1-2H3;1H/q+1;/p-1
InChI 密鑰
AZKSAVLVSZKNRD-UHFFFAOYSA-M
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相關類別
應用
噻唑蓝四唑蓝(MTT)可用于细胞增殖的检测。 MTT产生一种淡黄色的溶液，通过活细胞的线粒体脱氢酶将其转化为深蓝色、不溶于水的MTT福尔马赞。该蓝色晶体可溶于酸化异丙醇，可采用比色法测定其570 nm处的强度。
噻唑蓝四唑蓝(MTT)可用于细胞增殖的检测。 MTT产生一种淡黄色的溶液，通过活细胞的线粒体脱氢酶将其转化为深蓝色、不溶于水的MTT福尔马赞。该蓝色晶体可溶于酸化异丙醇，可采用比色法测定其570 nm处的强度。MTT已被用作组织化学/细胞化学试剂 以及用于检测NAD。 由于所使用的氰化物陷阱可与阳离子结合，组织中的ADP-连接酶系统无法采用MTT检测。 MTT迅速还原为福尔马赞，福尔马赞与镍、铜和钴螯合;钴螯合物已可用于氧化体系。
訊號詞
Warning
危險分類
Eye Irrit. 2 - Muta. 2 - Skin Irrit. 2 - STOT SE 3
標靶器官
Respiratory system
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
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Why can't a medium with phenol red be used? Is it because it interferes with MTT assay results? If after incubation with MTT, cells are washed with PBS and DMSO is used to dissolve the crystals, does this still apply?
1 answer-
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Should I prepare fresh for each use or I directly resuspend in 20 ml of water (for the 100 mg bottle) and store? In the latter case, how can I store the solution?
1 answer-
The solution may be filtered through a 0.2 μm filter and stored at 2-8°C for frequent use or stored in aliquots and frozen at -20°C for extended periods.
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What's the difference between M5655 and M2003?
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Product M5655 is Thiazolyl Blue Tetrazolium Bromide and Product M2003 is Thiazolyl blue formazan.
MTT (Cat. no. M5655) is used in the measurement of cell proliferation. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan (Product M2003) by mitochondrial dehydrogenases of living cells. The blue MTT formazan crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. Thus, the amount of blue formazan is proportional to the number of viable cells. For more information regarding this particular assay, we do offer an assay kit, TOX1, which is based on this principle: https://www.sigmaaldrich.com/product/sigma/tox1.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What type of multi-well plate should be used for the Thiazolyl Blue Tetrazolium Bromide (MTT) assay?
1 answer-
It is recommended that flat bottom multi-well plates be used for the MTT assay. The use of tissue culture treated plates is only important if necessary for good cell growth. If cells are grown in larger plates, the supernatant after cell lysis can be transferred to a 96 well plate for reading.
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What can be used to make a solution of Thiazolyl Blue Tetrazolium Bromide (MTT)?
1 answer-
We test the solubility of MTT in water at a concentration of 5 mg/mL. For the MTT viability assay, we dissolve Product No. M5655 at a concentration of 5 mg/ml in RPMI-1640 without phenol red. This medium is available as a powder (Product No.R8755) or liquid (Product No. R7509). MTT can be solubilized in any culture media or balanced salt solution that does not contain phenol red as a pH indicator.
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Is the MTT assay quantitative?
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The MTT asay is a method to assess cell viability. This assay is a semiquantitative assay. The assay is used to compare the viability changes in treated cells to untreated cells. The absorbance is indicative of the cell number. The higher the absorbance, the greater the number of viable cells present. Most researchers compare the absorbance of the two samples as a ratio (ABS treated cells/ABS untreated cells) to get a fold increase/decrease in cell number.
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How can Thiazolyl Blue Tetrazolium Bromide be used for staining dehydrogenase in tissue sections?
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Products M2128 or M5655, Thiazolyl Blue Tetrazolium Bromide can be used for staining dehydrogenase in tissue sections. See the method below. Reagent PreparationMTT (2 mg/ml distilled water) 2.5 ml0.2 M Tris buffer, pH 7.4 2.5 ml0.5 M cobalt chloride 0.5 ml0.05 M magnesium chloride 1.0 mlDistilled water 2.5 mlThe pH of this solution is adjusted to 7.0 if necessary. Aliquot and store at -20°C until required. Sample PreparationCut cytostat sections at 5 to 7 microns. Do not fix. Method:1. Incubate sections in appropriate incubation solution at 37°C for 30-60 minutes.2. Transfer sections to 15% formol saline, 15 minutes.3. Wash in distilled water4. Counterstain in 2% methyl green if required.5. Wash in distilled water.6. Mount in glycerin Jelly. ResultsEnzyme: Black formazan deposits with MTT Source: Bancroft and Gamble, Theory and Practice of Histological Techniques, Fifth Edition. Churchill Livingstone, London, 2002, page 610.
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Why is a reference wavelength used for data analysis of Thiazolyl Blue Tetrazolium Bromide (MTT)?
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The reference wavelength (630-690 nm) is used to adjust for the optical variation in the wells of the plate. It also corrects for dust and fingerprints that can be present. The assay is read at 570 nm. The reference wavelength needs to be far enough away from the assay wavelength so as not to affect those values.
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What can be used to solubilize MTT formazan (the product of the Thiazolyl Blue Tetrazolium Bromide - MTT reaction)?
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Mitochondrial dehydrogenases of viable cells cleave the tetrazolium ring of the MTT, yielding purple MTT formazan crystals. These crystals are not soluble in aqueous solutions; they may be dissolved in acidified isopropanol (0.1 N HCl in anhydrous isopropanol). If this does not lyse the cells, Triton X-100 can be added to the acidified isopropanol. A typical working concentration is 10% Triton X-100 (i.e., 1 volume of 100% Triton X-100 + 9 volumes of acidified isopropanol).
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