The MTT asay is a method to assess cell viability. This assay is a semiquantitative assay. The assay is used to compare the viability changes in treated cells to untreated cells. The absorbance is indicative of the cell number. The higher the absorbance, the greater the number of viable cells present. Most researchers compare the absorbance of the two samples as a ratio (ABS treated cells/ABS untreated cells) to get a fold increase/decrease in cell number.
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包裝
pkg of 1 kit
技術
UV/Vis spectroscopy: suitable
protein quantification: suitable
Amax
570 nm
應用
cell analysis
detection
檢測方法
colorimetric
運輸包裝
dry ice
儲存溫度
−20°C
一般說明
细胞生长测定试剂盒（基于MTT）设计用于以分光光度法测量细胞生长，作为活细胞中线粒体活性的函数。MTT(3-(4,5 -二甲基噻唑-2)- 2,5 -二苯基溴化四唑)系统是一种简单、准确、可重复性的方法，可通过线粒体脱氢酶活性测量活细胞活性。
應用
包裝
每个试剂盒包括5瓶试剂，每瓶含1 mL 5 mg/ml MTT的RPMI溶液（无酚红）和50 mL MTT溶剂（0.1N盐酸的无水异丙醇溶液）。 优化后的各组分体积大小尤其适合多孔板细胞生长。 MTT溶液按总培养体积的10%添加，因此每个试剂盒的测试次数取决于培养容量和/或体积大小。
相關產品
訊號詞
Danger
危險分類
Eye Irrit. 2 - Flam. Liq. 2 - Muta. 2 - STOT SE 3
標靶器官
Central nervous system
儲存類別代碼
3 - Flammable liquids
閃點（°F）
53.6 °F - closed cup
閃點（°C）
12 °C - closed cup
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品質控制指導方針，以維持高品質、經驗證且無污染的細胞培養物。免費下載 ECACC 手冊。
Quality control guidelines to maintain high quality authenticated and contamination-free cell cultures. Free ECACC handbook download.
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Is the MTT assay quantitative?
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What is the difference between Product No. CGD1, Cell Growth Determination Kit and Product No.TOX1, In Vitro Toxicology Assay Kit?
1 answer-
Product No. CGD1 and TOX1 are both MTT based assays. The major difference between Product No. CGD1 and TOX1 is that TOX1 contains 10 % Triton X-100 in the solubilization solution for better lysis of the cells and solubilization of the MTT. If there is complete cell lysis, the results obtained with both kits would be the same. The MTT in this kit is a solution, whereas the TOX1 assay kit contains the MTT as a powder that is solubilized before use.
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What are the components of the Product No. CGD1, Cell Growth Determination Kit and Product No.TOX1, In Vitro Toxicology Assay Kit?
1 answer-
CGD1 contains 5 vials of 5 mg/vial of MTT and 50 ml of MTT solvent (solubilization solution) and TOX1 contains 5 vials of 15 mg/vial of MTT and 125 ml of solubilization solution.
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What is the difference between product CGD1, Cell Growth Determination Kit and product TOX1, In Vitro Toxicology Assay Kit?
1 answer-
Product CGD1 and TOX1 are both MTT based assays. The major difference between product CGD1 and TOX1 is that TOX1 contains 10 % Triton X-100 in the solubilization solution for better lysis of the cells and solubilization of the MTT. If there is complete cell lysis, the results obtained with both kits would be the same. The MTT in this kit is a solution, whereas the TOX1 assay kit contains the MTT as a powder that is solubilized before use.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Can we use media with phenol red with Product CGD1, Cell Growth Determination Kit?
1 answer-
You most likely would not be able to use a medium containing phenol red with this kit. We have found that the phenol red can contribute to higher background, and therefore lower sensitivity.
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Is 10% fetal bovine serum (FBS) in the culture medium compatible with Product CGD1, Cell Growth Determination Kit?
1 answer-
High protein levels may form a precipitate when the MTT solubilization solution is added. Samples with protein concentrations equivalent to 10% FBS or 4 mg/mL protein seem acceptable.
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