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Sigma-Aldrich

体外毒理学检测试剂盒（基于MTT法）

同義詞:

mitochondrial activity assay

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.84

用途

 kit sufficient for 1,000 tests

包裝

pkg of 1 kit

儲存條件

dry at room temperature

λmax

570 nm

應用

cell analysis
detection

檢測方法

colorimetric

儲存溫度

2-8°C

相關類別

一般說明

本试剂盒旨在利用活细胞的线粒体活性功能通过比色法测定细胞数/细胞活力。MTT法是一种简便、准确、结果可重复的方法。关键组分是(3-[4,5-二甲基-2-噻唑基]-2,5-二苯基溴化四唑) 或MTT。溶于无酚红的培养基或平衡盐溶液中的MTT溶液呈浅黄色。其四唑环可被活细胞的线粒体脱氢酶剪切而产生不溶于水溶液的紫色甲臜晶体。在570nm波长处测量转化染料的吸光度。

應用

MTT体外细胞毒性检测试剂盒已用于通过(3-[4,5-二甲基-2-噻唑基]-2,5-二苯基溴化四唑)MTT法检测金纳米颗粒(AuNP)的细胞毒性。它还用于检测SH-SY5Y培养物经过6-羟基多巴胺(6-OHDA)处理后的细胞活性。

生化／生理作用

将MTT转化成水不溶性的有色甲臜衍生物，然后用酸性异丙醇溶解。

訊號詞

Danger

危險分類

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 2 - Muta. 2 - Skin Corr. 1 - STOT SE 3

標靶器官

Central nervous system, Respiratory system

儲存類別代碼

3 - Flammable liquids

閃點（°F）

53.6 °F

閃點（°C）

12 °C

分析證明 (COA)

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J Carmichael et al.
Cancer research, 47(4), 936-942 (1987-02-15)
Drug sensitivity assays were performed using a variation of a colorimetric [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)] assay on V79, CHO-AuxB1, CHRC5, NCI-H460, and NCI-H249 cell lines following optimization of experimental conditions for each cell line. Results from this assay were compared with
F Denizot et al.
Journal of immunological methods, 89(2), 271-277 (1986-05-22)
A convenient way to estimate the number of viable cells growing in microtitre tray wells is to use a colorimetric assay and an automatic microplate scanning spectrophotometer. One such assay, developed by Mosmann, depends on the reduction by living cells
Off-resonance plasmonic enhanced femtosecond laser optoporation and transfection of cancer cells
Baumgart J, et al.
Biomaterials, 33(7), 2345-2350 (2012)
Dalia Chávez-García et al.
Journal of biomedical materials research. Part B, Applied biomaterials, 108(6), 2396-2406 (2020-02-06)
Luminescent lanthanide downconversion nanoparticles (DCNPs) provide a combination of high luminescence intensity, sharp emission peaks with narrow bandwidth and a large Stokes' shift, leading to high-performance biomedical applications mainly for imaging. The purpose of this study is to present a
Jun Wang et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 17(2), 343-351 (2008-12-11)
Spliceosome-mediated RNA trans-splicing has emerged as an exciting mode of RNA therapy. Here we describe a novel trans-splicing strategy, which targets highly abundant pre-mRNAs, to produce therapeutic proteins in vivo. First, we used a pre-trans-splicing molecule (PTM) that mediated trans-splicing
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