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WTA1

TransPlex® Whole Transcriptome Amplification Kit

DNA polymerase separate.

Synonim(y):

Transcriptome Amplification Kit

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Gabaryty przesyłkiSKUDostępnośćCena netto
50 reactions
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
14 860,00 zł

Informacje o tej pozycji

NACRES:
NA.55
UNSPSC Code:
41121800

14 860,00 zł

Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
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Pomoc techniczna
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Pozwól nam pomóc

Quality Level

200

technique(s)

whole genome amplification: suitable

dilution

(WTA)

input

purified DNA

shipped in

wet ice

storage temp.

−20°C

General description

TransPlex®, a Whole Transcriptome Amplification (WTA) method, allows for representative amplification of nanogram quantities of total RNA in less than 4 hours without 3′-bias. Microgram quantities of amplification product generated from tissue, cultured cells, formalin-fixed samples, or serum are suitable for downstream applications such as qPCR and microarray analyses.

Application

TransPlex® Whole Transcriptome Amplification Kit has been used to synthesize double-stranded cDNA. It has also been used in the amplification of cDNA.[1]
Suitable for use with downstream applications including:
  • qPCR
  • microarray analysis
  • cloning

Biochem/physiol Actions

The WTA process involves two steps. In the first step, sample RNA is reverse transcribed with non-self-complementary primers composed of a quasi-random 3′ end and a universal 5′ end. As polymerization proceeds, displaced single strands serve as new templates for primer annealing and extension. The resultant OmniPlex cDNA library, comprised of random, overlapping 100 - 1000 base fragments flanked by universal end sequence, is then amplified by PCR with the universal primer to produce WTA product.

Features and Benefits

  • Amplification of total RNA in less than 4 hours with less than 30 minutes of "hands-on" time
  • Only 5 ng of starting material required to produce a highly representative library from total RNA
  • Microgram quantities of amplification product generated from intact RNA from tissue, cultured cells, serum, or degraded RNA from formalin-fixed paraffin-embedded samples

Legal Information

TransPlex is a registered trademark of Rubicon Genomics, Inc.
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SEQRWTA2SEQXE
TransPlex® Whole Transcriptome Amplification Kit DNA polymerase separate.

Sigma-Aldrich

WTA1

TransPlex® Whole Transcriptome Amplification Kit

SeqPlex RNA Amplification Kit For use with high throughput sequencing technologies

Sigma-Aldrich

SEQR

SeqPlex RNA Amplification Kit

Complete Whole Transcriptome Amplification Kit DNA polymerase included, Complete Kit with optimized enzyme to amplify total RNA in <4 hours, no 3′ bias

Sigma-Aldrich

WTA2

Complete Whole Transcriptome Amplification Kit

SeqPlex DNA Amplification Kit For use with high throughput sequencing technologies, Whole Genome Amplification kit designed to facilitate Next Gen Sequencing.

Sigma-Aldrich

SEQXE

SeqPlex DNA Amplification Kit

technique(s)

whole genome amplification: suitable

technique(s)

whole genome amplification: suitable

technique(s)

whole genome amplification: suitable

technique(s)

whole genome amplification: suitable

input

purified DNA

input

purified RNA

input

purified RNA

input

purified DNA

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

dilution

(WTA)

dilution

(WTA)

dilution

(WTA)

dilution

(WGA)

Elementy zestawu są też dostępne oddzielnie

Numer produktu
Opis
Karta charakterystyki & Cennik
  • W4502
    Water, Nuclease-Free Water, for Molecular Biology
    Karta charakterystyki
  • D7295
    Deoxynucleotide Mix, 10 mM, Molecular Biology Reagent
    Karta charakterystyki

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H319

Hazard Classifications

Eye Irrit. 2

flash_point_f

Not applicable

flash_point_c

Not applicable

wgk

WGK 3

Klasa składowania

12 - Non Combustible Liquids

Wybierz jedną z najnowszych wersji:

Certyfikaty analizy (CoA)

Lot/Batch Number
SLCF9667
SLCC3063
SLBZ6319
SLBQ6830V
SLBP1558V

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Jeśli potrzebujesz konkretnej wersji, możesz wyszukać konkretny certyfikat według numeru partii lub serii.

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Protokoły

Integration of Sigma® TransPlex® with Agilent Microarray

TransPlex® kits' amplification products integrate seamlessly into existing Agilent workflows for microarray target expression analyses.

Powiązane treści

Whole Transcriptome Amplification FAQs

Transplex Whole Transcriptome Amplification FAQs on topics including whole transcriptome steps, RNA source, including archival fixed tissue, library purification, quantitation of the product and downstream applications

Product Information Sheet - WTA1

Instructions

Sara Planamente et al.
Molecular microbiology, 86(5), 1085-1099 (2012-10-10)
GABA acts as an intercellular signal in eukaryotes and as an interspecies signal in host-microbe interactions. Structural characteristics of selective eukaryotic GABA receptors and bacterial GABA sensors are unknown. Here, we identified the selective GABA-binding protein, called Atu4243, in the
Ki Wook Kim et al.
Open forum infectious diseases, 6(2), ofz025-ofz025 (2019-03-01)
The importance of gut bacteria in human physiology, immune regulation, and disease pathogenesis is well established. In contrast, the composition and dynamics of the gut virome are largely unknown; particularly lacking are studies in pregnancy. We used comprehensive virome capture
Anne H Rowley et al.
The Journal of infectious diseases, 203(7), 1021-1030 (2011-03-16)
Intracytoplasmic inclusion bodies (ICI) have been identified in ciliated bronchial epithelium of Kawasaki disease (KD) patients using a synthetic antibody derived from acute KD arterial IgA plasma cells; ICI may derive from the KD etiologic agent. Acute KD bronchial epithelium
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Numer pozycji handlu globalnego

SKUNUMER GTIN
WTA1-50RXN04061835378807

Questions

1–6 of 6 Questions  

  1. Can TransPlex® Whole Transcriptome Amplification Kit, Product WTA1, be used for downstream applications?

    1 answer

    1. Upon creation of a cDNA library, quantitative qPCR or microarray analysis may be performed

      Helpful?

  2. What is the concentration of the dNTPs supplied with the TransPlex® Whole Transcriptome Amplification Kit, Product WTA1?

    1 answer

    1. The dNTP Mix (D6193) is supplied as 10 mM each.

      Helpful?

  3. What is the difference between WTA and WGA?   I use the TransPlex® Whole Transcriptome Amplification Kit, Product WTA1.

    1 answer

    1. The starting material for WTA is RNA, whereas for WGA it is genomic DNA.  After the RNA is reverse-transcribed into the DNA complement by WTA, the OmniPlex library is formed. This library is in turn amplified by PCR.

      Helpful?

  4. Can TransPlex® Whole Transcriptome Amplification Kit, Product WTA1, be used to amplify archival fixed tissue or degraded samples?

    1 answer

    1. Yes, the WTA kit effectively amplifies degraded RNA, including formalin-fixed and paraffin-embedded samples.

      Helpful?

  5. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  6. Is the TransPlex® Whole Transcriptome Amplification Kit, Product WTA1, also applicable for ribosomal RNA and/or small RNA amplification?

    1 answer

    1. Using the kit as is, Ribosomal RNA does appear to amplify. However, we have not quantitated how well it is amplified.

      Helpful?

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