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Sigma-Aldrich

SeqPlex RNA Amplification Kit

For use with high throughput sequencing technologies

Synonim(y):

WGA kit, Whole transcriptome amplification

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4540,00 zł
50 REACTIONS
22 240,00 zł
500 REACTIONS
103 590,00 zł

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10 REACTIONS
4540,00 zł
50 REACTIONS
22 240,00 zł
500 REACTIONS
103 590,00 zł

About This Item

Kod UNSPSC:
41121800
NACRES:
NA.55

4540,00 zł


Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności

Poziom jakości

metody

whole genome amplification: suitable

Warunki transportu

wet ice

temp. przechowywania

−20°C

Opis ogólny

The SeqPlex RNA Amplification Kit for whole transcriptome amplification (WTA) is designed to facilitate next-generation sequencing (NGS) from small quantities or from degraded/highly fragmented RNA (e.g. RNA from formalin-fixed paraffin-embedded (FFPE) tissue samples). The SeqPlex kit allows the user to pre-amplify these and other small quantity/highly fragmented RNA samples for input into a NGS workflow. It also facilitates the amplification of non-polyA tailed RNA isolated from tissue, cultured cells, formalin-fixed samples, or serum while maintaining patterns of differential expression found in the unamplified sample.

Zastosowanie

SeqPlex RNA Amplification Kit has been used to amplify poly(A)-selected RNA.[1][2]

Cechy i korzyści

  • Low quantities of total RNA random priming technology amplifies fragmented or intact RNA from all sources including FFPE and RIP.
  • Semi-degenerate library primer design for more complete transcriptome coverage and efficient priming.
  • No need to fragment DNA before sequencing.
  • Amplifies ds-cDNA in 8 hours or less.
  • Compatible with all next generation sequencing platforms except Pacific Bioscience.

Inne uwagi

SEQR-500RXN is manufactured on-demand. Contact us for more information.

Informacje prawne

SeqPlex is a trademark of Sigma-Aldrich Co. LLC
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Kod klasy składowania

11 - Combustible Solids

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Grégory Seumois et al.
Nature immunology, 15(8), 777-788 (2014-07-07)
A characteristic feature of asthma is the aberrant accumulation, differentiation or function of memory CD4(+) T cells that produce type 2 cytokines (TH2 cells). By mapping genome-wide histone modification profiles for subsets of T cells isolated from peripheral blood of
Kathryn D Tuttle et al.
Nature communications, 9(1), 2650-2650 (2018-07-10)
During development in the thymus, invariant natural killer T (iNKT) cells commit to one of three major functionally different subsets, iNKT1, iNKT2, and iNKT17. Here, we show that T cell antigen receptor (TCR) signal strength governs the development of iNKT
Giuliana P Mognol et al.
Proceedings of the National Academy of Sciences of the United States of America, 114(13), E2776-E2785 (2017-03-12)
T-cell exhaustion is a progressive loss of effector function and memory potential due to persistent antigen exposure, which occurs in chronic viral infections and cancer. Here we investigate the relation between gene expression and chromatin accessibility in CD8+ tumor-infiltrating lymphocytes
Meritxell Jodar et al.
Human reproduction update, 19(6), 604-624 (2013-07-17)
BACKGROUND Spermatozoa are highly differentiated, transcriptionally inert cells characterized by a compact nucleus with minimal cytoplasm. Nevertheless they contain a suite of unique RNAs that are delivered to oocyte upon fertilization. They are likely integrated as part of many different
Isaac Engel et al.
Nature immunology, 17(6), 728-739 (2016-04-19)
Natural killer T cells (NKT cells) have stimulatory or inhibitory effects on the immune response that can be attributed in part to the existence of functional subsets of NKT cells. These subsets have been characterized only on the basis of

Protokoły

The SeqPlex RNA Amplification kit provides a method for amplification of total RNA or isolated mRNA prior to entry into the workflows of the commonly used deep sequencing platforms.

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