WTA2

Complete Whole Transcriptome Amplification Kit

DNA polymerase included, Complete Kit with optimized enzyme to amplify total RNA in <4 hours, no 3′ bias

• Synonim(y): transcriptome amplification kit
• NACRES: NA.55
• UNSPSC Code: 41121800

Właściwości

• Nazwa produktu: Complete Whole Transcriptome Amplification Kit, DNA polymerase included, Complete Kit with optimized enzyme to amplify total RNA in <4 hours, no 3′ bias
• technique(s): whole genome amplification: suitable
• shipped in: wet ice
• storage temp.: −20°C
• Quality Level: 200

Opis

Application

Complete Whole Transcriptome Amplification Kit is used for the following applications:

• To establish a protocol for the simultaneous analysis of DNA and RNA viruses present in pig feces using process controlled deep sequencing.[1]
• Reverse transcription and cDNA amplification[2][3][4][5][6][7]
• For the synthesis and amplification of cDNA library using Genomic RNA released from immunocaptured PPV particles[8]
• Nucleic Acid Preparation and Deep Sequencing (The extracted nucleic acids were randomly primed for cDNA synthesis)[9]
• Amplification of RNA andssDNA viruses for Viral metagenomics from low-input clinical samples[10]

Suitable for use with downstream applications including:

• qPCR
• microarray analysis
• cloning

Biochem/physiol Actions

The WTA2 process involves two steps. In the first step, sample RNA is reverse transcribed with non-self-complementary primers composed of a quasi-random 3′ end and a universal 5′ end. During this process, displaced single strands serve as new templates for primer annealing and extension. The resultant cDNA library, comprised of random, overlapping 100 - 1000 base fragments flanked by universal end sequence. The 2nd step amplifies the cDNA library by PCR using WTA2 polymerase and a universal end primer to produce WTA2 product.

Features and Benefits

• Achieve up to 10,000x amplification in less than 4 hours with less than 30 minutes of "hands on" time required
• Only 20 pg of total RNA template is required to amplify suitable cDNA for microarray profiling
• Contains all needed components for cDNA amplification
• Achieve linear amplification of expressed genes and exons without 3′ or 5′ bias
• Effectively amplifies single cell or low input RNA, including mRNA and total RNA from any animal, plant, or microorganism

General description

WTA2 is optimized to amplify RNA from formalin-fixed, paraffin-embedded (FFPE) and other damaged or degraded samples. Whole Transcriptome Amplification (WTA) technology, allows for representative amplification of low nanogram quantities of total RNA in less than 4 hours without 3′-bias. Amplification products are suitable for applications such as qPCR, micro array analysis, and cloning. The WTA2 kit contains the polymerase needed to amplify the cDNA library.

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Elementy zestawu są też dostępne oddzielnie

• Library Synthesis Enzyme
• Library Synthesis Solution
• Amplification Mix
• Library Synthesis Buffer
• W4502 Water, Nuclease-Free Water, for Molecular Biology
• Amplification Enzyme
• D7295 Deoxynucleotide Mix, 10 mM, Molecular Biology Reagent

Informacja o środkach bezpieczeństwa

• Klasa składowania: 10 - Combustible liquids