WGA polymerase is compatible with TA cloning with the following alteration to the PCR step: Be sure to include a 7 to 30 minute extension at 72°C after the last cycle to ensure that all PCR products are full length and 3´ adenylated.
Kluczowe dokumenty
WGA2
GenomePlex® Complete Whole Genome Amplification (WGA) Kit
Optimized kit with enzyme for amplifying a variety of DNA including FFPE tissue
Synonim(y):
Whole genome amplification kit
Wybierz wielkość
959,00 zł
Wybierz wielkość
About This Item
959,00 zł
Polecane produkty
Poziom jakości
metody
whole genome amplification: suitable
Warunki transportu
wet ice
temp. przechowywania
−20°C
Powiązane kategorie
Opis ogólny
Zastosowanie
Cechy i korzyści
- Higher yield from minimal template: Amplification of nanogram amounts of genomic DNA to microgram yields (around 10 μg) in less than about three hoursrs
- Nanograms of samples can be preserved at –20 °C for future use
- Choose from a variety of DNA sources: whole blood, buccal swab, blood card, plant, soil, & formalin-fixed paraffin-embedded tissue (FFPE)
- Whole-genome amplification (WGA) DNA polymerase increases the amplification accuracy
- Whole-genome representation with no detectable allele bias
- Compatible with many downstream applications such as TaqMan® assays, single nucleotide polymorphism (SNP) analysis, comparative genomic hybridization (CGH) analysis
Inne uwagi
Informacje prawne
Elementy zestawu są też dostępne oddzielnie
- W4502Water, Nuclease-Free Water, for Molecular BiologyKarta charakterystyki
produkt powiązany
Hasło ostrzegawcze
Danger
Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Klasyfikacja zagrożeń
Resp. Sens. 1
Kod klasy składowania
12 - Non Combustible Liquids
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Wybierz jedną z najnowszych wersji:
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Klienci oglądali również te produkty
Produkty
aCGH technology evolves for high-res chromosomal analysis, needing a large DNA input per sample, advancing genetic research.
aCGH technology enhances chromosomal analysis, requiring significant DNA input per sample.
Technologia aCGH usprawnia analizę chromosomów, wymagając znacznego wkładu DNA na próbkę.
Quality DNA crucial for meaningful data; intact DNA key for successful chromosomal aberration analysis from FFPE tissues.
Protokoły
GenomePlex® amplification improves DNA yield from soil samples, ensuring downstream analysis reliability despite potential nuclease damage.
Amplifikacja GenomePlex® poprawia wydajność DNA z próbek gleby, zapewniając niezawodność dalszej analizy pomimo potencjalnych uszkodzeń nukleazy.
Whole genome amplification (WGA) of plasma and serum DNA presents a unique challenge due to the small amount of nucleic acid in such samples.
Whole Genome Amplification products, including kits for DNA extraction, support various DNA sources.
-
Is the WGA2 GenomePlex® Amplification Kit polymerase compatible with TA cloning?
1 answer-
Helpful?
-
-
If starting with fragmented DNA, what is the smallest size fragment which can be successfully amplified with Product WGA2, GenomePlex® Amplification Kit, and do I still need to do the fragmentation step?
1 answer-
The kit works best for fragments 400 bp and larger. When starting with fragmented DNA, we recommend: (1) skipping the fragmentation heat step, although the buffer should be added, and (2) increasing the PCR cycles from 14 to 20.
Helpful?
-
-
Can I use Product WGA2, GenomePlex® Amplification Kit, to amplify DNA from a single cell?
1 answer-
Using this kit to amplify DNA from a single cell is not recommended. We recommend using the GenomePlex Single Cell WGA Kit (WGA4) for such application. WGA4 was developed for use with single cells and includes an optimized cell lysis protocol which has been incorporated into the fragmentation step.
Helpful?
-
-
What is the major difference between WGA1 and WGA2 kits?
1 answer-
Functionally, WGA1 and WGA2 kits are identical. The only difference between the two kits is that WGA2 is supplied with the WGA polymerase.
Helpful?
-
-
Can I amplify single stranded DNA with WGA2, GenomePlex® Amplification Kit?
1 answer-
When starting with single stranded starting materials, we recommend (1) skipping the fragmentation heat step, although the buffer should be added and (2) increasing the PCR cycles from 14 to 20. Note that if the ssDNA is actually cDNA from polyadenylated RNA, the kit will likely not give good representation of the input material, as the poly(T) ends constitute a large, non-random fraction.
Helpful?
-
-
What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
-
Active Filters
Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.
Skontaktuj się z zespołem ds. pomocy technicznej