SAE0094

SAvPhire^™ Monomeric Streptavidin

High-affinity biotin binding—reduces aggregation and enables precise, reliable detection and purification of biotinylated molecules

• Synonim(y): mSA3
• UNSPSC Code: 12352202
• NACRES: NA.25
• Form: lyophilized powder
• Recombinant: expressed in E. coli

Właściwości

• Nazwa produktu: SAvPhire^™ Monomeric Streptavidin, recombinant, expressed in E. coli, High Affinity
• recombinant: expressed in E. coli
• form: lyophilized powder
• shipped in: wet ice
• storage temp.: −20°C

Opis

General description

Sequence contains N-terminal 6×-Histidine-tag and a C-terminal FLAG-tag

Biochem/physiol Actions

SAvPhire^™ Monomeric Streptavidin is a variant of the biotin-binding streptavidin, which naturally exists as a tetramer. Many monomeric streptavidin proteins have rapid dissociation kinetics, which prevent their use in stable labeling applications. However, SAvphire^™ Monomeric Streptavidin has been engineered to have a dissociation rate comparable to that of tetrameric streptavidin.

The size and monovalency of this product provide advantages over tetrameric streptavidin in applications such as functional cell surface labeling and atomic force spectroscopy. These advantages include:

• Minimizing distribution or dynamics in the probing of biotinylated targets
• Reducing risk of aggregation, precipitation, and cross-linking in pre-incubation steps with biotinylated molecules
• Easier access to desired target

This product can be used in traditional biotin-binding applications, including Western blotting, immunoprecipitation, immunohistochemistry, and flow cytometry.

Preparation Note

Once reconstituted, the solution can be stored at 2-8 °C for at least 1 month. For long term storage, aliquot and store at -20 °C.

This product is lyophilized from PBS, pH 7.4. Reconstitute the vial contents to a 4 mg/mL solution by adding water as follows:

• 1 mg vial: add 250 μL of water for a 4 mg/mL solution
• 5 mg vial: add 1.25 mL of water for a 4 mg/mL solution

The reconstituted product can be further diluted in water or PBS as needed.

Legal Information

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

SAvPhire is a trademark of Sigma-Aldrich Co. LLC

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable