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Merck

S5442

Sigma-Aldrich

SyntheChol® NS0 Supplement

SyntheChol® NS0 Supplement

500 ×, synthetic cholesterol, animal component-free, aqueous solution, sterile-filtered, suitable for cell culture

Synonim(y):

Cholesterol solution

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2 ML
233,00 zł
10 ML
825,00 zł

233,00 zł


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2 ML
233,00 zł
10 ML
825,00 zł

About This Item

Wzór empiryczny (zapis Hilla):
C27H46O
Numer CAS:
Masa cząsteczkowa:
386.65
Numer MDL:
Kod UNSPSC:
12352211
eCl@ss:
39023139
Identyfikator substancji w PubChem:
NACRES:
NA.75

233,00 zł


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pochodzenie biologiczne

non-animal source

sterylność

sterile-filtered

linia produktu

BioXtra

Formularz

aqueous solution

stężenie

500 ×

metody

cell culture | mammalian: suitable

temp. przechowywania

2-8°C

ciąg SMILES

[H][C@@]12CC=C3C[C@@H](O)CC[C@]3(C)[C@@]1([H])CC[C@]4(C)[C@H](CC[C@@]24[H])[C@H](C)CCCC(C)C

InChI

1S/C27H46O/c1-18(2)7-6-8-19(3)23-11-12-24-22-10-9-20-17-21(28)13-15-26(20,4)25(22)14-16-27(23,24)5/h9,18-19,21-25,28H,6-8,10-17H2,1-5H3/t19-,21+,22+,23-,24+,25+,26+,27-/m1/s1

Klucz InChI

HVYWMOMLDIMFJA-DPAQBDIFSA-N

informacje o genach

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Zastosowanie

SyntheChol® NS0 Supplement is used as media supplement for NS0 mouse myeloma cell growth and the growth of other cholesterol-dependent cell lines.[1][2][3][4][5]

Cechy i korzyści

Synthetic, non-animal cholesterol solution that is designed as a supplement to our hybridoma media formulations to promote excellent cell growth and protein productivity for NS0 mouse myeloma and other cholesterol-dependent cell lines.

Informacje prawne

SyntheChol is a registered trademark of Merck KGaA, Darmstadt, Germany
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produkt powiązany

Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


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Certyfikaty analizy (CoA)

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Arthur Nathan Brodsky et al.
Methods (San Diego, Calif.), 56(3), 408-417 (2011-10-29)
DNA microarray analysis of gene expression has become a valuable tool for bioprocessing research aimed at improving therapeutic protein yields. The highly parallel nature of DNA microarray technology allows researchers to assess hundreds of gene simultaneously, essentially enabling genome-wide snapshots.
Suba Dharshanan et al.
Cytotechnology, 66(4), 625-633 (2013-07-25)
From our recent publications, it was found that the deimmunization method (Dharshanan et al. (2012) Sci Res Essays 7:2288-2299) should be applied for the development of humanized anti-C2 monoclonal antibody (H1C2 mAb). However, the overlapping-PCR mutagenesis procedure used to insert
Steve Schoonooghe et al.
BMC biotechnology, 9, 70-70 (2009-08-13)
Tumour associated antigens on the surface of tumour cells, such as MUC1, are being used as specific antibody targets for immunotherapy of human malignancies. In order to address the poor penetration of full sized monoclonal antibodies in tumours, intermediate sized
Marco Guadagnoli et al.
Blood, 117(25), 6856-6865 (2011-05-06)
APRIL (A proliferation-inducing ligand) is a TNF family member that binds two TNF receptor family members, TACI and BCMA. It shares these receptors with the closely related TNF family member, B-cell activating factor (BAFF). Contrary to BAFF, APRIL binds heparan
Nancy Lynn Booth et al.
Cancer immunology, immunotherapy : CII, 58(8), 1229-1244 (2008-12-18)
We have developed a high-throughput screen (HTS) to search for novel molecules that can synergize with TRAIL, thus promoting apoptosis of ACHN renal tumor cells in a combinatorial fashion. The HTS detects synthetic compounds and pure natural products that can

Produkty

Discover answers to your frequently asked questions about animal component-free, xenobiotic-free, and chemically defined media and reagents for cell culture.

Questions

1–4 of 4 Questions  
  1. What is the concentration of product S5442?

    1 answer
    1. Unfortunately, the formulation of Product S5442 is considered proprietary.

      Helpful?

  2. Is this formulation stable at room temperature? If so, how long is it stable?

    1 answer
    1. This product ships at ambient temperature and is stable for relatively short periods (up to two weeks for transit time) at room temperature. However, refrigerated temperatures are recommended for long-term storage.
      Please see the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/397/094/s5442dat.pdf

      Helpful?

  3. Could you please clarify the concentration of cholesterol in this product both at the original 500X solution and at the final 1X?

    1 answer
    1. The concentration of synthetic cholesterol in this product is 0.25% or 2.5 mg/mL as supplied. The product is considered a 500X preparation meaning that 2 mL of this solution will supplement 1 L of culture medium. The 1X concentration, when diluted in 1L of medium, would be 5 ug/mL. Some cells may require a higher concentrations of cholesterol. The suggested usage range for this product is 2 - 6 mL per liter of medium. See the link below to review the product datasheet.
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/397/094/s5442dat.pdf

      Helpful?

  4. Hi, What is the concentration of Synthechol in this product in mg/ml or mol/l Thank you

    1 answer
    1. Unfortunately, the formulation of item S5442 is proprietary. The concentration of the synthetic cholesterol is 0.25% (w/v) as supplied.

      Helpful?

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