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S5442

SyntheChol® NS0 Supplement

500 ×, synthetic cholesterol, animal component-free, sterile-filtered, aqueous solution, suitable for cell culture

SyntheChol® NS0 Supplement

Synonim(y):

Cholesterol solution

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Do Państwa/SKUDostępnośćCena netto
2 mL
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242,00 zł
10 mL
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858,00 zł

Informacje o tej pozycji

Wzór empiryczny (zapis Hilla):
C27H46O
Numer CAS:
57-88-5
Masa cząsteczkowa:
386.65
UNSPSC Code:
12352211
eCl@ss:
39023139
PubChem Substance ID:
24899653
NACRES:
NA.75
MDL number:
MFCD00003646

242,00 zł

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biological source

non-animal source

Quality Segment

500

sterility

sterile-filtered

product line

BioXtra

form

aqueous solution

concentration

500 ×

technique(s)

cell culture | mammalian: suitable

storage temp.

2-8°C

SMILES string

[H][C@@]12CC=C3C[C@@H](O)CC[C@]3(C)[C@@]1([H])CC[C@]4(C)[C@H](CC[C@@]24[H])[C@H](C)CCCC(C)C

InChI

1S/C27H46O/c1-18(2)7-6-8-19(3)23-11-12-24-22-10-9-20-17-21(28)13-15-26(20,4)25(22)14-16-27(23,24)5/h9,18-19,21-25,28H,6-8,10-17H2,1-5H3/t19-,21+,22+,23-,24+,25+,26+,27-/m1/s1

InChI key

HVYWMOMLDIMFJA-DPAQBDIFSA-N

Gene Information

human ... POLA1(5422), TOP2A(7153)
rat ... Ar(24208), Polb(29240)

Application

SyntheChol® NS0 Supplement is used as media supplement for NS0 mouse myeloma cell growth and the growth of other cholesterol-dependent cell lines.[1][2][3][4][5]

Features and Benefits

Synthetic, non-animal cholesterol solution that is designed as a supplement to our hybridoma media formulations to promote excellent cell growth and protein productivity for NS0 mouse myeloma and other cholesterol-dependent cell lines.

Legal Information

SyntheChol is a registered trademark of Merck KGaA, Darmstadt, Germany
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Ta pozycja
C3045C8503137672
SyntheChol® NS0 Supplement 500 ×, synthetic cholesterol, animal component-free, sterile-filtered, aqueous solution, suitable for cell culture

Sigma-Aldrich

S5442

SyntheChol® NS0 Supplement

Cholesterol powder, BioReagent, suitable for cell culture, ≥99%

Sigma-Aldrich

C3045

Cholesterol

Cholesterol from sheep wool, ≥92.5% (GC), powder

Sigma-Aldrich

C8503

Cholesterol

SyntheChol® Synthetic Cholesterol For Cell Culture, EMPROVE® EXPERT

SAFC

137672

SyntheChol® Synthetic Cholesterol

biological source

non-animal source

biological source

ovine wool

biological source

sheep wool

biological source

synthetic

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

-

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

room temp

storage temp.

15-25°C

form

aqueous solution

form

powder

form

powder

form

-

concentration

500 ×

concentration

-

concentration

-

concentration

-

Quality Level

500

Quality Level

200

Quality Level

200

Quality Level

500

Klasa składowania

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certyfikaty analizy (CoA)

Lot/Batch Number

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Questions

1–4 of 4 Questions  

  1. What is the concentration of product S5442?

    1 answer

    1. Unfortunately, the formulation of Product S5442 is considered proprietary.

      Helpful?

  2. Is this formulation stable at room temperature? If so, how long is it stable?

    1 answer

    1. This product ships at ambient temperature and is stable for relatively short periods (up to two weeks for transit time) at room temperature. However, refrigerated temperatures are recommended for long-term storage.
      Please see the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/397/094/s5442dat.pdf

      Helpful?

  3. Could you please clarify the concentration of cholesterol in this product both at the original 500X solution and at the final 1X?

    1 answer

    1. The concentration of synthetic cholesterol in this product is 0.25% or 2.5 mg/mL as supplied. The product is considered a 500X preparation meaning that 2 mL of this solution will supplement 1 L of culture medium. The 1X concentration, when diluted in 1L of medium, would be 5 ug/mL. Some cells may require a higher concentrations of cholesterol. The suggested usage range for this product is 2 - 6 mL per liter of medium. See the link below to review the product datasheet.
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/397/094/s5442dat.pdf

      Helpful?

  4. Hi, What is the concentration of Synthechol in this product in mg/ml or mol/l Thank you

    1 answer

    1. Unfortunately, the formulation of item S5442 is proprietary. The concentration of the synthetic cholesterol is 0.25% (w/v) as supplied.

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