The cell pellet can be stored at -80°C with a small amount of PBS, avoiding storage with culture medium. Upon thawing, it's recommended to wash the cells once with PBS and then remove the PBS. Following this step, the assay buffer should be used for extraction according to the kit instructions. It's important to avoid multiple freeze-thaw cycles. However, the supplier does not specify the maximum time frozen cells will remain stable for assay.
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MAK078
Xanthine Oxidase Activity Assay Kit
sufficient for 100 colorimetric or fluorometric tests
Synonim(y):
XO Activity Assay Kit
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About This Item
Kod UNSPSC:
12161503
NACRES:
NA.84
Polecane produkty
zastosowanie
sufficient for 100 colorimetric or fluorometric tests
metoda wykrywania
colorimetric
fluorometric
powiązane choroby
rheumatological diseases; gastrointestinal diseases; cardiovascular diseases
temp. przechowywania
−20°C
informacje o genach
human ... XDH(7498)
mouse ... XDH(22436)
rat ... XDH(497811)
Opis ogólny
Xanthine Oxidase (XO) catalyzes the sequential oxidation of hypoxanthine to xanthine, and xanthine to uric acid and hydrogen peroxide. In humans and other primates, XO controls the final step of purine catabolism and is normally found in the liver and the intestinal mucosa. In rodents, XO is broadly expressed in most tissues. While XO activity is normally very low in blood, liver injury can result in the release of XO into blood. XO may contribute to the pathogenesis of gout and cardiovascular disease, and XO activity or expression may be upregulated in these conditions.
Zastosowanie
Przydatność
Suitable for the measurement of xanthine oxidase in a variety of samples including tissue, cells and serum
Zasada
The Xanthine Oxidase Activity Assay Kit provides a simple and direct procedure for measuring XO activity in a variety of biological samples. XO activity is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/ fluorometric (λex = 535/λem = 587 nm) product, proportional to the hydrogen peroxide generated. One unit of XO is defined as the amount of enzyme that catalyzes the oxidation of xanthine, yielding 1.0 μmole of uric acid and hydrogen peroxide per minute at 25 °C.
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10 - Combustible liquids
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Xanthine oxidase is an important enzyme of purine catabolism pathway and has been associated directly in pathogenesis of gout and indirectly in many pathological conditions like cancer, diabetes and metabolic syndrome. In this research rutin, a bioactive flavonoid was explored
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Does the kit allow the use of cell pellets that have been frozen at -80°C, or do the samples need to be fresh, considering that the cells should be rapidly homogenized with the assay buffer?
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