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GE17-5280-01

nProtein A Sepharose 4 Fast Flow

Cytiva 17-5280-01, pack of 5 mL

Synonim(y):

Sepharose 4 Fast Flow

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About This Item

Kod UNSPSC:
41106500
NACRES:
NA.56

ligand

native protein A (S. aureus)

opakowanie

pack of 5 mL

producent / nazwa handlowa

Cytiva 17-5280-01

warunki przechowywania

(20% Ehtanol)

macierz

4% cross-linked agarose

średnia średnica

90 μm (d50v)

cleaning

2-10

working range

3-9

pojemność

>30 mg binding capacity(human IgG/ml)

przydatność

suitable for bioprocess medium

temp. przechowywania

2-8°C

Powiązane kategorie

Opis ogólny

nProtein A Sepharose 4 Fast Flow is native protein A coupled to Sepharose 4 Fast Flow for the purification of monoclonal and polyclonal antibodies at both laboratory and process scale.

nProtein A Sepharose 4 Fast Flow is native protein A coupled to the well established Sepharose 4 Fast Flow base matrix. The native protein A ligand is produced by fermenting a selected strain of Staphylococcus aureus. The purified protein is coupled to the cross-linked 4% agarose base matrix by the cyanogen bromide technique, giving a highly stable medium with minimal non-specific adsorption. nProtein A Sepharose 4 Fast Flow is manufactured without using animal-derived components.

nProtein A Sepharose 4 Fast Flow has nearly twice the total IgG binding capacity of Protein A Sepharose CL-4B, and is suitable for recovery and purification of monoclonal antibodies from cell culture at both laboratory and process scale. nProtein A Sepharose 4 Fast Flow was developed and tested in co-operation with leading manufacturers of purified monoclonal antibody products, and is used in routine commercial production.

As member of the BioProcess media range, nProtein A Sepharose 4 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

Cechy i korzyści

  • Replaces Protein A Sepharose 4 Fast Flow, the first Cytiva Protein A medium for large-scale purification of antibodies.
  • Used in routine commercial production of monoclonal antibodies
  • Free from animal-derived components.

Przechowywanie i stabilność

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Komentarz do analizy

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informacje prawne

Sepharose is a trademark of Cytiva
This page may contain text that has been machine translated.

Piktogramy

Flame

Hasło ostrzegawcze

Warning

Zwroty wskazujące rodzaj zagrożenia

Zwroty wskazujące środki ostrożności

Kod klasy składowania

3 - Flammable liquids


Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Eunice Cho et al.
Cell reports, 34(13), 108928-108928 (2021-04-01)
Flux through the RAF-MEK-ERK protein kinase cascade is shaped by phosphatases acting on the core components of the pathway. Despite being an established drug target and a hub for crosstalk regulation, little is known about dephosphorylation of MEK, the central
Sylwia Jones et al.
Scientific reports, 10(1), 663-663 (2020-01-22)
Antibody combinations targeting cell surface receptors are a new modality of cancer therapy. The trafficking and signalling mechanisms regulated by such therapeutics are not fully understood but could underlie differential tumour responses. We explored EGFR trafficking upon treatment with the

Produkty

This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.

Na tej stronie przedstawiono różne opcje oczyszczania dla nośników do chromatografii z sefarozą Protein A oraz opisano typowe warunki wiązania i elucji dla nośników do chromatografii z sefarozą Protein A.

This page describes immunoprecipitation (immunoaffinity or pull-down techniques).

Protokoły

This page provides information about different pull-down assays for the further isolation of multiprotein complexes to identify their components with products from Cytiva.

Ta strona zawiera informacje o różnych testach pull-down do dalszej izolacji kompleksów wielobiałkowych w celu identyfikacji ich składników za pomocą produktów Cytiva.

Protein A, coupled to Sepharose, binds IgG via its Fc regions, facilitating affinity chromatography purification.

This page shows how to separate IgG antibodies by affinity chromatography using Protein G Sepharose 4 Fast Flow from Cytiva.

Zobacz wszystko

Powiązane treści

Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.

Badanie interakcji białko-białko in vitro za pomocą testów pull-down, z wykorzystaniem metod powinowactwa, GST pull-down, TAP i koimmunoprecypitacji.

Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.

Skontaktuj się z zespołem ds. pomocy technicznej