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B6149
5-Bromo-4-chloro-3-indolyl phosphate disodium salt
chromogenic, ≥98% (HPLC), powder
Synonim(y):
Sól disodowa 5-BCIP
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50 MG
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100 MG
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8810,00 zł
271,00 zł
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Zmień widok
25 MG
271,00 zł
50 MG
293,00 zł
100 MG
455,00 zł
500 MG
1500,00 zł
1 G
2200,00 zł
5 G
8810,00 zł
About This Item
Wzór liniowy:
C8H4BrClNO4P · 2Na
Numer CAS:
Masa cząsteczkowa:
370.43
Numer MDL:
Kod UNSPSC:
12352204
Identyfikator substancji w PubChem:
NACRES:
NA.83
271,00 zł
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Nazwa produktu
5-Bromo-4-chloro-3-indolyl phosphate disodium salt, ≥98% (HPLC)
Próba
≥98% (HPLC)
Formularz
powder
rozpuszczalność
H2O: 20 mg/mL
DMF: insoluble
temp. przechowywania
−20°C
ciąg SMILES
[Na+].[Na+].[O-]P([O-])(=O)Oc1c[nH]c2ccc(Br)c(Cl)c12
InChI
1S/C8H6BrClNO4P.2Na/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;;/h1-3,11H,(H2,12,13,14);;/q;2*+1/p-2
Klucz InChI
OAZUOCJOEUNDEK-UHFFFAOYSA-L
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Zastosowanie
5-Bromo-4-chloro-3-indolyl phosphate disodium salt has been used to detect protein-antibody complexes.[1] It has also been used for tissue staining in alkaline phosphatase histochemistry.[2]
For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry.
BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.
BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.
For the colorimetric detection of alkaline phosphatase-labeled molecules.
Substraty
Histochemical substrate for alkaline phosphatase.
Informacje prawne
BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany
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Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Środki ochrony indywidualnej
Eyeshields, Gloves, type N95 (US)
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Produkty
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
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