16670
5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt
≥95.0% (HPLC), powder
Synonim(y):
BCIP® p-toluidine salt, X-phosphate p-toluidine salt
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Wybierz wielkość
100 MG
537,20 zł
1 G
3330,00 zł
537,20 zł
Cena katalogowa632,00 złZaoszczędź 15%Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Informacje o tej pozycji
Wzór empiryczny (zapis Hilla):
C8H6BrClNO4P · C7H9N
Numer CAS:
Masa cząsteczkowa:
433.62
NACRES:
NA.83
PubChem Substance ID:
UNSPSC Code:
12352204
EC Number:
229-506-1
MDL number:
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Pozwól nam pomócNazwa produktu
5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt, ≥95.0% (HPLC)
InChI
1S/C8H6BrClNO4P.C7H9N/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;1-6-2-4-7(8)5-3-6/h1-3,11H,(H2,12,13,14);2-5H,8H2,1H3
InChI key
QEIFSLUFHRCVQL-UHFFFAOYSA-N
SMILES string
Cc1ccc(N)cc1.OP(O)(=O)Oc2c[nH]c3ccc(Br)c(Cl)c23
assay
≥95.0% (HPLC)
form
powder
storage temp.
2-8°C
Quality Level
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Ta pozycja | |||
|---|---|---|---|
| assay ≥95.0% (HPLC) | assay ≥99% | assay ≥98% (HPLC) | assay ≥99% (HPLC) |
| form powder | form powder | form powder | form powder |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
General description
Histochemical substrate for alkaline phosphatase.
Application
For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry.
BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.
BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.
For the colorimetric detection of alkaline phosphatase-labeled molecules.
Legal Information
BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany
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Klasa składowania
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
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