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52225
Alkyne MegaStokes dye 673
for copper catalyzed click labeling
Synonim(y):
4-{2-[7-(Diethylamino)-2-oxo-2H-1-benzopyran-3-yl]ethenyl}-1-(4-pentyn-1-yl)-3-sulfo-pyridinium inner salt
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About This Item
Wzór empiryczny (zapis Hilla):
C25H26N2O5S
Masa cząsteczkowa:
466.55
Numer MDL:
Kod UNSPSC:
12352200
Identyfikator substancji w PubChem:
NACRES:
NA.32
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Formularz
solid
fluorescencja
λex 542 nm; λem 673 nm in ethanol
temp. przechowywania
−20°C
ciąg SMILES
CCN(CC)c1ccc2C=C(\C=C\c3cc[n+](CCC#C)cc3S([O-])(=O)=O)C(=O)Oc2c1
InChI
1S/C24H24N2O5S/c1-4-7-13-25-14-12-18(23(17-25)32(28,29)30)8-9-20-15-19-10-11-21(26(5-2)6-3)16-22(19)31-24(20)27/h1,8-12,14-17H,5-7,13H2,2-3H3
Klucz InChI
ODMGQNUCRFIQIN-UHFFFAOYSA-N
Opis ogólny
The MegaStokes dyes for „click" biological labeling, licensed by LuminoChem are virtually covering the whole visible spectrum reaching the infra-red regime. Besides dyes that are being capable of participating in classical copper catalyzed 1,3-dipolar cycloaddition reaction with the counterparting function, we also provide dyes containing cyclooctyne moiety an alkyne derivative that enables copper free clicking to azides.
These dyes are noteworthy for their large Stokes-shift (120 or 125 nm). Dyes for this kind are exceptionally useful in fluorescence resonance electron transfer (FRET) applications as they do not interfere with the spectral bands of the second fluorophore a common problem in FRET technology. Azido sugars have been used for click-labeling of surface glycoproteins, recently. We have adapted this system to demonstrate the ability of our dyes to undergo bioorthogonal labeling reaction, efficiently. Prior to labeling, Chinese hamster ovary (CHO) cells were incubated with these MegaStockesdyes to test the possible cytostatic effects of these dyes. Experimental results have shown that neither dye is toxic up to 50 μM concentration.
These dyes are noteworthy for their large Stokes-shift (120 or 125 nm). Dyes for this kind are exceptionally useful in fluorescence resonance electron transfer (FRET) applications as they do not interfere with the spectral bands of the second fluorophore a common problem in FRET technology. Azido sugars have been used for click-labeling of surface glycoproteins, recently. We have adapted this system to demonstrate the ability of our dyes to undergo bioorthogonal labeling reaction, efficiently. Prior to labeling, Chinese hamster ovary (CHO) cells were incubated with these MegaStockesdyes to test the possible cytostatic effects of these dyes. Experimental results have shown that neither dye is toxic up to 50 μM concentration.
Zastosowanie
Barwnik Alkyne MegaStokes 673 jest odczynnikiem stosowanym do katalizowanego miedzią znakowania typu "click".
Opakowanie
Bottomless glass bottle. Contents are inside inserted fused cone.
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The alkyne-azide Cu(I)-catalyzed Huisgen cycloaddition, a click-type reaction, was used to label a double-stranded oligonucleotide (siRNA) with fluorine-18. An alkyne solid support CPG for the preparation of monostranded oligonucleotides functionalized with alkyne has been developed. Two complementary azide labeling agents
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