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11426320001
Roche
Anti-Fluorescein
from mouse IgG1 (clone B13-DE1)
Synonim(y):
antibody
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About This Item
Kod UNSPSC:
12352200
Polecane produkty
pochodzenie biologiczne
mouse
białko sprzężone
unconjugated
forma przeciwciała
purified immunoglobulin
rodzaj przeciwciała
primary antibodies
klon
B13-DE1, monoclonal
Formularz
lyophilized
opakowanie
pkg of 100 μg
producent / nazwa handlowa
Roche
izotyp
IgG1
temp. przechowywania
2-8°C
Opis ogólny
Monoclonal antibody to fluorescein.
The detection of bound antibody can be carried out directly in one step using an anti-mouse Ig fluorochrome/enzyme conjugate, or in a two-step procedure with anti-mouse Ig fluorescein and, subsequently, anti-fluorescein enzyme conjugate.
The antibody does not contain any protein and can hence be used for coating and labeling.
Contents
Lyophilizate, stabilized
The detection of bound antibody can be carried out directly in one step using an anti-mouse Ig fluorochrome/enzyme conjugate, or in a two-step procedure with anti-mouse Ig fluorescein and, subsequently, anti-fluorescein enzyme conjugate.
The antibody does not contain any protein and can hence be used for coating and labeling.
Contents
Lyophilizate, stabilized
Specyficzność
The monoclonal antibody reacts with free and bound fluorescein.
Zastosowanie
Use Anti-Fluorescein for the detection of fluorescein-labeled compounds using:
- ELISA
- Immunohistocytochemistry
- In situ hybridization
- Western blot
Uwaga dotycząca przygotowania
Working concentration: Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline:
Working solution: Phosphate buffered saline, pH 7.4
Using water instead the antibody can precipitate.
- ELISA: 2 to 4 μg/ml
- Immunohistocytochemistry: 0.5 to 2 μg/ml
- In situ hybridization: 0.2 to 0.4 μg/ml
- Western blot: 0.5 to 2 μg/ml
Working solution: Phosphate buffered saline, pH 7.4
Using water instead the antibody can precipitate.
Rekonstytucja
Add 1 ml double-distilled water to a final concentration of 100 μg/ml.
Reconstitute for 15 minutes.
Reconstitute for 15 minutes.
Inne uwagi
For life science research only. Not for use in diagnostic procedures.
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Hasło ostrzegawcze
Warning
Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Klasyfikacja zagrożeń
Aquatic Chronic 3 - Skin Sens. 1
Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 2
Temperatura zapłonu (°F)
does not flash
Temperatura zapłonu (°C)
does not flash
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