MAB131871

Anti-KLH Antibody, clone 3B1

clone 3B1, from mouse

• Synonim(y): Keyhole Limpet Hemocyanin
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: mouse
• Poziom jakości: 100
• forma przeciwciała: purified immunoglobulin
• rodzaj przeciwciała: primary antibodies
• klon: 3B1, monoclonal
• reaktywność gatunkowa: keyhole limpet, mollusk
• metody: western blot: suitable
• izotyp: IgMκ
• numer dostępu UniProt: P81732; Q10583; Q10584
• Warunki transportu: ambient
• docelowa modyfikacja potranslacyjna: unmodified

Opis

Opis ogólny

Substances with low molecular weights are generally not immunogenic and require the aid of carrier proteins to stimulate an immune response. Carrier proteins have relatively high molecular weights and carry many epitopes. An immune response to a hapten-carrier conjugate results in antibodies that are specific for both the hapten and the carrier. Hemocyanin is a high molecular weight respiratory metalloprotein found in the hemolymph of many mollusks and crustaceans. Molluscan hemocyanin is based on an approximately 400 kDa polypeptide subunit that is folded into a chain of multiple, immunologically distinct domains, each with a binuclear copper site. Keyhole Limpet Hemocyanin (KLH), the hemocyanin from the giant keyhole limpet, Megathura crenulata, is the most widely used carrier protein. It is an extremely large protein (6 - 7.5 million daltons) that can exist as a di-decamer, multi-decamers, or flexible tubules of varying length. It is easily dissociated into subunits and the dissociation-association reaction depends on protein concentration, ionic strength, and pH. At pH 6.5, KLH exists in a fully associated state without any evidence of dissociation products. In contrast, at pH 8.5 in borate saline the molecule is completely dissociated.

Specyficzność

Clone 3B1 detects keyhole limpet hemocyannin (KLH) and KLH conjugated to peptide or protein.

Immunogen

Purified Keyhole limpet hemocyanin from Megathura crenulata

Zastosowanie

Anti-KLH, clone 3B1, Cat. No. MAB131871, is a mouse monoclonal antibody that detects Keyhole limpet hemocyanin and has been tested for use in Western Blotting.

Research Category
Inflammation & Immunology

Jakość

Evaluated by Western Blotting with Keyhole Limpet Hemocyanin (KLH).

Western Blotting Analysis: A 1:500 dilution of this antibody detected Keyhole Limpet Hemocyanin.

Opis wartości docelowych

~375 kDa observed; Calculated ~ 6 to 7.5 MDa (undissociated). Uncharacterized bands may be observed in some lysate(s).

Postać fizyczna

Format: Purified

Purified mouse monoclonal antibody IgM in PBS with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Inne uwagi

Concentration: Please refer to lot specific datasheet.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 2