ABE476

Anti-GAL4 Antibody (activation domain)

from rabbit, purified by affinity chromatography

• Synonim(y): Regulatory protein GAL4
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: rabbit
• Poziom jakości: 100
• forma przeciwciała: affinity isolated antibody
• rodzaj przeciwciała: primary antibodies
• klon: polyclonal
• oczyszczone przez: affinity chromatography
• reaktywność gatunkowa: yeast
• metody: western blot: suitable
• numer dostępu NCBI: NP_015076
• numer dostępu UniProt: P04386
• Warunki transportu: wet ice
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: Saccharomyces cerevisiae ... Gal4(855828)

Opis

Opis ogólny

GAL4 is a transcriptional activator which controls the expression of genes involved in the catabolism of galactose. In addition to a DNA-binding domain, Gal4 contains two acidic regions: activating region I (aa148-238) and activating region II (aa768-881), which can both activate transcription when independently joined to the DNA-binding domain. The activation domains of Gal4 have been shown to interact with Gal80, TBP, SUG1, and ADA2, among other general transcription factors.

Specyficzność

This antibody recognizes the C-terminus of GAL4 (activation domain).

Immunogen

Epitope: C-terminus

GST-tagged recombinant protein corresponding to the C-terminus of yeast GAL4 (activation domain).

Zastosowanie

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Use Anti-GAL4 (activation domain) Antibody (Rabbit Polyclonal Antibody) validated in WB to detect GAL4 (activation domain) also known as Regulatory protein GAL4.

Jakość

Evaluated by Western Blot in Gal4 positive and Gal4 negative yeast cell lysate.

Western Blot Analysis: A 1:160,000 dilution of this antibody detected Gal4 in 5 mg of Gal4 positive (+ recombinant Gal4) yeast cell lysate. This antibody also detected small mounts of endogenous Gal4 in Gal4 negative (- recombinant Gal4) yeast cell lysate.

Opis wartości docelowych

~28 kDa, ~38 kDa, ~77 kDa observed

Powiązanie

Replaces: 06-283

Postać fizyczna

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Komentarz do analizy

Control
Gal4 positive and Gal4 negative yeast

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable