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580560

TCEP, Hydrochloride

A water-soluble and odorless disulfide-reducing agent that is more stable and effective than DTT.

Synonim(y):

TCEP, Hydrochloride, tris(2-Carboxyethyl)phosphine, HCl

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Informacje o tej pozycji

Wzór empiryczny (zapis Hilla):
C9H15O6P · xHCl
Numer CAS:
Masa cząsteczkowa:
250.19 (free base basis)
UNSPSC Code:
12352128
NACRES:
NA.25
MDL number:
Assay:
≥98% (elemental analysis)
Form:
crystalline solid
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Quality Segment

assay

≥98% (elemental analysis)

form

crystalline solid

reaction suitability

reagent type: reductant

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, desiccated

color

white

solubility

water: 5 mg/mL

shipped in

ambient

storage temp.

2-8°C

SMILES string

P(CCC(=O)[O-])(CCC(=O)[O-])CCC(=O)[O-]

InChI

1S/C9H15O6P/c10-7(11)1-4-16(5-2-8(12)13)6-3-9(14)15/h1-6H2,(H,10,11)(H,12,13)(H,14,15)/p-3

InChI key

PZBFGYYEXUXCOF-UHFFFAOYSA-K

General description

A water-soluble and odorless disulfide reducing agent which is more stable and effective than DTT. TCEP reduces even the most stable water-soluble alkyl disulfides at pH 4.5 within 5 min. TCEP is also capable of functioning as a reducing agent even in pH range of 1.5-8.5. Is more resistant to air-oxidation as compared to DTT.

Application


  • Peptide and Protein Desulfurization with Diboron Reagents.: This study investigates the use of TCEP hydrochloride in peptide and protein desulfurization, demonstrating its effectiveness in facilitating diboron-mediated reactions. (Jing & Walczak, 2024).

  • A Study on Thiol-Michael Addition to Semi-Synthetic Elastin-Hyaluronan Material for Electrospun Scaffolds.: TCEP hydrochloride is utilized to enhance the thiol-Michael addition reaction in the development of electrospun scaffolds, contributing to advanced biomaterials research. (Laezza et al., 2024).

  • Optimized total RNA isolation from bovine sperm with enhanced sperm head lysis.: This paper presents an optimized protocol for RNA isolation from bovine sperm, highlighting the role of TCEP hydrochloride in improving sperm head lysis efficiency. (Tiwari et al., 2024).

  • Target Recognition-Triggered Peroxidase-Mimicking Activity Depression in Homochiral Nanochannels for Identifying Cystine Enantiomers.: The study uses TCEP hydrochloride in a novel peroxidase-mimicking system to differentiate cystine enantiomers, showcasing its application in chiral analysis. (Xu et al., 2023).

  • In situ digestion of alcohol-fixed cells for quantitative proteomics.: TCEP hydrochloride is applied in the in situ digestion of fixed cells to enhance the accuracy of quantitative proteomics analyses. (Hatano et al., 2023).

Packaging

Packaged under inert gas

Other Notes

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Corrosive (L)
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Ta pozycja
580567C470675259
description

A water-soluble and odorless disulfide-reducing agent that is more stable and effective than DTT.

description

-

description

powder

description

BioUltra, ≥98% (NMR)

assay

≥98% (elemental analysis)

assay

≥98% (GC)

assay

-

assay

≥98% (NMR)

solubility

water: 5 mg/mL

solubility

water: 50 mg/mL

solubility

H2O: 50 mg/mL

solubility

H2O: 1 M, clear, colorless (no residue)

Quality Level

100

Quality Level

100

Quality Level

300

Quality Level

300

reaction suitability

reagent type: reductant

reaction suitability

reagent type: reductant

reaction suitability

reagent type: reductant

reaction suitability

reagent type: reductant

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

form

crystalline solid

form

solid

form

powder

form

crystals


pictograms

Corrosion

signalword

Danger

hcodes

Hazard Classifications

Eye Dam. 1 - Skin Corr. 1B

Klasa składowania

8A - Combustible corrosive hazardous materials

wgk

WGK 1



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Questions

1–2 of 2 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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