68957

Tris(2-carboxyethyl)phosphine hydrochloride

BioUltra, suitable for electrophoresis, SDS-PAGE tested

• Synonim(y): TCEP.HCL, TCEP
• Wzór empiryczny (zapis Hilla): C₉H₁₅O₆P · HCl
• Numer CAS: 51805-45-9
• Masa cząsteczkowa: 286.65
• UNSPSC Code: 12352128
• NACRES: NA.32
• PubChem Substance ID: 329761275
• MDL number: MFCD00145469
• Beilstein/REAXYS Number: 3724376
• Assay: 97.5-102.5%
• Form: powder

Właściwości

• product line: BioUltra
• Quality Segment: 100
• assay: 97.5-102.5%
• form: powder
• mol wt: 286.65 g/mol
• concentration: 90-100 % (w/w) (Tris(2-carboxyethyl)phosphine hydrochloride)
• technique(s): electrophoresis: suitable
• color: white
• mp: 175-177 °C
• solubility: H₂O: soluble
• density: 1.041 g/cm³ at 25 °C
• suitability: SDS-PAGE tested
• SMILES string: Cl[H].OC(=O)CCP(CCC(O)=O)CCC(O)=O
• InChI: 1S/C9H15O6P.ClH/c10-7(11)1-4-16(5-2-8(12)13)6-3-9(14)15;/h1-6H2,(H,10,11)(H,12,13)(H,14,15);1H
• InChI key: PBVAJRFEEOIAGW-UHFFFAOYSA-N

Opis

General description

Tris(2-carboxyethyl) phosphine hydrochloride (TCEP.HCL ) is a useful reducing agent for Sulphur and nitrogen-containing compounds. TCEP.HCL and the resultant oxide are water-soluble. [1] TCEP.HCL has electron-rich phosphorus and oxygen atoms to combine with various metals, including Rh, Ir, and Pd. It rapidly complexes with Rh and Ir in dioxane at ambient temperature.[2] It reduces disulfide linkages in a range of peptides and proteins in biological disulfide.[1]

Application

TCEP.HCL has been used in various bioconjugation applications, both in vitro and in vivo, to tag proteins and live organisms with great effectiveness under mild physiological circumstances.[3] TCEP.HCL is suitable for varied biological and synthetic applications. At an acidic pH, TCEP.HCL can be used to convert organic disulfides to thiols in water irreversibly.[1]

Water soluble reagent, used for selective reduction of disulfides. More stable than DTT and useful in mass spectrometry applications.

Features and Benefits

The product has the following benefits:

• TCEP.HCL lowers reactive oxygen species levels.[3]
• It increases glutathione levels and mitochondrial content.[3]
• It has anti-cancer effects.[2]

Analysis Note

agarose gel electrophorese corresponds to requirements

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Informacja o środkach bezpieczeństwa

• pictograms: GHS05
• signalword: Danger
• hcodes: H314
• pcodes: P260 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363
• Hazard Classifications: Eye Dam. 1 - Skin Corr. 1B
• Klasa składowania: 8A - Combustible corrosive hazardous materials
• wgk: WGK 1