The minimum purity of this product is 95% as determined by SDS-PAGE. The purity is confirmed in the Certificate of Analysis. Please see the link below to review a lot-specific Certificate:
https://www.sigmaaldrich.com/product/mm/324681#product-documentationZ#product-documentation
324681
Elastase, Human Neutrophil
Elastase, Human Neutrophil, CAS 9004-06-2, is a serine protease that degrades elastin, collagen, and proteoglycans
Synonim(y):
Elastase, Human Neutrophil
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| Gabaryty przesyłki | SKU | Dostępność | Cena netto |
|---|---|---|---|
| 50 μg | Dostępne do wysyłki DZISIAJzKuehne + Nagel Sp. z o.o. | 2030,00 zł | |
| 100 μg | Dostępne do wysyłki DZISIAJzKuehne + Nagel Sp. z o.o. | 3590,00 zł | |
| 1 mg | Przewidywany termin wysyłki22 maja 2026zKuehne + Nagel Sp. z o.o. | 27 850,00 zł |
Informacje o tej pozycji
Numer CAS:
UNSPSC Code:
12352204
NACRES:
NA.77
MDL number:
Specific activity:
≥20 units/mg protein
Pomoc techniczna
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Pozwól nam pomócQuality Level
description
Merck USA index - 14, 3535
form
lyophilized solid
specific activity
≥20 units/mg protein
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
shipped in
ambient
storage temp.
−20°C
General description
Elastase, Human Neutrophil, CAS 9004-06-2, is a serine protease that degrades elastin, collagen, and proteoglycans
Native elastase from human neutrophil. A serine protease that degrades elastin, collagen, and proteoglycans. Its overexpression has been implicated in the development of pulmonary emphysema, rheumatoid arthritis, and cystic fibrosis.
Native elastase from human neutrophil. Degrades collagen, elastin, and proteoglycans. Elastase has been implicated in the development of pulmonary emphysema and rheumatoid arthritis.
Physical form
Salt-free.
Preparation Note
Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Acidic stock solutions are stable for up to 1 month at 4°C or for up to 1 year at -20°C.
Prepared from blood that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
Reconstitute in 50 mM sodium acetate, pH 5.5, with 200 mM NaCl or in other aqueous buffers. Material is most active near a neutral pH, but to maintain stability upon reconstitution, use in a slightly acidic buffer (pH 5.5) to avoid autolysis.
Analysis Note
Major band by SDS-PAGE
Other Notes
One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol of MeO-Suc-Ala-Ala-Pro-Val-pNA (Cat. No. 454454) per min at 25°C, pH 8.0.
Siedle, B., et al. 2002. Biorg. Med. Chem.10, 2855.
Junger, W.G., et al. 1992. Biol. Chem. Hoppe-Seyler 373, 691.
Selak, M.A. 1992. Thromb. Haemost.68, 570.
Baugh, R.J., and Travis, J. 1976. Biochemistry15, 836.
Junger, W.G., et al. 1992. Biol. Chem. Hoppe-Seyler 373, 691.
Selak, M.A. 1992. Thromb. Haemost.68, 570.
Baugh, R.J., and Travis, J. 1976. Biochemistry15, 836.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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Ta pozycja | |||
|---|---|---|---|
| specific activity ≥20 units/mg protein | specific activity ≥50 units/mg protein (Bradford) | specific activity ≥250 units/mg protein | specific activity ≥25 units/mg protein |
| form lyophilized solid | form lyophilized powder | form lyophilized | form lyophilized |
| storage condition OK to freeze | storage condition - | storage condition OK to freeze | storage condition OK to freeze, desiccated |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| shipped in ambient | shipped in dry ice | shipped in ambient | shipped in ambient |
| manufacturer/tradename Calbiochem® | manufacturer/tradename - | manufacturer/tradename Calbiochem® | manufacturer/tradename Calbiochem® |
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Klasa składowania
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
Certyfikaty analizy (CoA)
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Powiązane treści
Numer pozycji handlu globalnego
| SKU | NUMER GTIN |
|---|---|
| 324681-100UG | 07790788059583 |
| 324681-50UG | 04055977196825 |
| 324681-1MG | 04055977196795 |
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Could you provide the purity data of item Elastase, Human Neutrophil, cat# 324681? Thank you.
1 answer-
Helpful?
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How do I calculate how much enzyme powder I need to dissolve to hydrolyze the 290 mg of protein contained in the solution?
1 answer-
I am not sure which substrate you are using. The unit definition for this product is One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol of MeO-Suc-Ala-Ala-Pro-Val-pNA (Cat. No. 454454) per min at 25°C, pH 8.0.
This product has unit activity specification as ≥20 units/mg protein. If for example the results are
20 units/mg, this means to use 1 unit you will need to use 50 ug of the elastase.
You might wish to refer to the Elastase activity assay at:Helpful?
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Hi Is this form of neutrophil elastase active and suitable be used directly in a cell assay? And is it sterile ? Mvh Magdalena
1 answer-
This form of neutrophil elastase is a salt-free preparation and is supplied in an active form with a specific activity of ≥20 units/mg protein. It is not sterile and has not been tested for cell culture applications. To use it in a cell culture assay, sterile filtration is necessary, which may result in some product retention on the filter.
Helpful?
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Has this product been tested for the presence of endotoxin?
1 answer-
Unfortunately, this product has not been tested for the presence for endotoxins
Helpful?
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what is the molecular wight of the enzyme ?
1 answer-
Unfortunately, the molecular weight has not been determined for this product. A number of estimates have been reported in the literature. Please see the link below to a publication that may be helpful:
https://www.sciencedirect.com/science/article/pii/S1016847823175091?via%3DihubHelpful?
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Is this the active form of the enzyme? or does it require activation by cathepsin?
1 answer-
Here are the recommended reconstitution and reaction conditions:
Solubility information:
Reconstitute in 50 mM sodium acetate, pH 5.5, with 200 mM NaCl or in other aqueous buffers. Material is most active near a neutral pH, but to maintain stability upon reconstitution, use in a slightly acidic buffer (pH 5.5) to avoid autolysis.
Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Acidic stock solutions are stable for up to 1 month at 4°C or for up to 1 year at -20°C.Human Neutrophil Elastase Protocol
1. Dissolve enzyme in reaction buffer: 100 mM Tris-HCl, pH 7.5, 500 mM NaCl.
2. Prepare the substrate stock solution by dissolving Elastase Substrate I (MeOSuc-Ala-Ala-Pro-Val-pNA, Cat. No. 324696) at 50 mM in DMSO.
3. Just prior to assay, dilute the substrate stock solution with an equal volume of 200 mM Tris-HCl, pH 8.0. Mix well.
4. Prepare the assay mixture by combining 960 µl of reaction buffer with 40 µl of diluted substrate stock solution.
5. Add enzyme to assay mixture, mix, and incubate at 25°C.
6. Monitor the change in absorbance at 410 nm. Use an ε of 8800 at 410 nm to calculate the hydrolysis of nitroanilide.Helpful?
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