219373
Cathepsin G, Human Neutrophil
Cathepsin G, Human Neutrophil, CAS 107200-92-0, is a purified native cathepsin G. Acts as a potent agonist of human platelet activation leading to their aggregation.
Synonim(y):
Cathepsin G, Human Neutrophil
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About This Item
Numer CAS:
Numer MDL:
Kod UNSPSC:
12352202
NACRES:
NA.77
Polecane produkty
pochodzenie biologiczne
human neutrophils
Poziom jakości
Próba
≥95% (SDS-PAGE)
Postać
lyophilized solid (Salt-free)
aktywność właściwa
≥2 units/mg protein
producent / nazwa handlowa
Calbiochem®
warunki przechowywania
OK to freeze
metody
inhibition assay: suitable
przydatność
suitable for molecular biology
Zastosowanie
life science and biopharma
Warunki transportu
ambient
temp. przechowywania
−20°C
informacje o genach
human ... CTSG(1511)
Opis ogólny
Research area: Cell Signaling
Cathepsin G is stored in its active form in azurophil granules of neutrophils or monocytes. Its mature form contains one potential glycan-binding site and three disulfide bonds.
Cathepsin G is stored in its active form in azurophil granules of neutrophils or monocytes. Its mature form contains one potential glycan-binding site and three disulfide bonds.
Zastosowanie
Cathepsin G, Human Neutrophil has been used to determine the in vitro bactericidal activities of cathepsin G and for in vitro digestion of plasma/acute wound fluids.
Działania biochem./fizjol.
This protease cleaves the triple-helix structure of type I collagen in a restricted manner and degrades nonhelical regions of type I and II collagens, fibronectin, aggrecan, and elastin. Additionally, cathepsin G activates ProMMP-3 and -8. Cathepsin G serves as a chemoattractant for human phagocytes and enhances the random motility of T cells. Additionally, it initiates chemotaxis, activates ERK1/2 and p38 MAPK, and facilitates the translocation of PKCζ to the cell membrane, without inducing a detectable calcium flux. Other biological functions of cathepsin G include: ECM components and plasma proteins degradation, bactericidal actions, cleavage of inflammatory mediators, conversion of angiotensin I to angiotensin II, platelet activation and induction of airway submucosal gland secretion.
Ostrzeżenie
Toxicity: Standard Handling (A)
Definicja jednostki
One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol of Suc-AAPF-pNA per min at 25°C, pH 7.5.
Uwaga dotycząca przygotowania
Prepared from blood that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
Reconstitute in 150 mM NaCl, 50 mM sodium acetate buffer, pH 5.5.
Reconstitute in 150 mM NaCl, 50 mM sodium acetate buffer, pH 5.5.
Rekonstytucja
Following reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 1 week at 4°C or for up to 6 months at -70°C.
Reconstitute in 50 mM NaOAc and 150 mM NaCl, pH 5.5.
Inne uwagi
Glusa, E., and Adam C. 2001. Por. J. Pharmacol.133, 422.
Shamamian, P., et al. 2001. J. Cell Physiol.189, 197.
Groutas, W.C., et al. 1993. Biochem. Biophys. Res. Commun.197, 730.
Stone, P.J., et al. 1993. Biochem. Biophys. Res. Commun.197, 130.
Groutas, W.C., et al. 1992. Arch. Biochem. Biophys.294, 144.
Maison, C.M., et al. 1991. J. Immunol.147, 921.
Travis, J. 1988. Am. J. Med.84, 37.
Shamamian, P., et al. 2001. J. Cell Physiol.189, 197.
Groutas, W.C., et al. 1993. Biochem. Biophys. Res. Commun.197, 730.
Stone, P.J., et al. 1993. Biochem. Biophys. Res. Commun.197, 130.
Groutas, W.C., et al. 1992. Arch. Biochem. Biophys.294, 144.
Maison, C.M., et al. 1991. J. Immunol.147, 921.
Travis, J. 1988. Am. J. Med.84, 37.
Informacje prawne
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
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