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05-1085

Sigma-Aldrich

Anti-IRS1 Antibody, clone 4.2.2

clone 4.2.2, from mouse

Synonim(y):

insulin receptor substrate 1

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About This Item

Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

pochodzenie biologiczne

mouse

Poziom jakości

100

forma przeciwciała

purified antibody

rodzaj przeciwciała

primary antibodies

klon

4.2.2, monoclonal

reaktywność gatunkowa

pig, canine, human, mouse, rat, monkey, bovine

metody

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

izotyp

IgG2aκ

numer dostępu NCBI

NP_005535.1

numer dostępu UniProt

P35568

Warunki transportu

wet ice

docelowa modyfikacja potranslacyjna

unmodified

informacje o genach

bovine ... Irs1(538598)
dog ... Irs1(486148)
human ... IRS1(3667)
mouse ... Irs1(16367)
pig ... Irs1(100512686)
rat ... Irs1(25467)
rhesus monkey ... Irs1(707870)

Opis ogólny

IRS1 (Insulin Receptor Substrate 1) transmits insulin signals via metabolic and mitogenic pathways. IRS1 is heavily phosphorylated on both serine and tyrosine residues. These phosphorylated tyrosines enable IRS to act as a docking protein that binds SH2 domains of such proteins as PI3 Kinase (phosphatidylinositol 3-kinase) and GRB2, resulting in activation. Over expression and phosphorylation of serine is associated with insulin resistance and breast cancer. Some of the more notable phosphorylation sites are Ser302 that is phosphorylated following insulin stimulation. Ser307, phosphorylated by JNK and IKK, is a key regulatory site that appears to disrupt the IRS1/IR interaction and inhibits insulin-mediated activation of the PI3 kinase and MAPK pathways, and Ser636/639 that is known to be phosphorylated by p70S6K downstream of mTOR and acts as a negative feedback loop.

Specyficzność

Predicted to cross-react with many other species based on 100% sequence homology with immunogen.
This antibody recognizes IRS1.

Immunogen

Synthetic peptide corresponding to amino acids 431-446 of mouse IRS1.

Zastosowanie

This Anti-IRS1 Antibody, clone 4.2.2 is validated for use in WB, IP, IC for the detection of IRS1.

Jakość

Evaluated by western blot on IR/IRS1 transfected CHO +/- Insulin lysate.

Western Blot Analysis:
1:1,000 dilution of this antibody was used to detect IRS1 in IRS/IR transfected CHO -/+ Calyculin A/ Okadaic Acid-treated cell lysate.

Opis wartości docelowych

~185 kDa

Postać fizyczna

Format: Purified
Purified mouse monoclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4),150 mM NaCl with 0.05% sodium azide.

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
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Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Certyfikaty analizy (CoA)

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Xihong Zhang et al.
Endocrinology, 164(3) (2023-01-08)
Targeting the type I insulin-like growth factor receptor (IGF-IR) has not been successful in breast cancer. Data suggest the highly homologous insulin receptor (IR) may be an alternate growth stimulatory pathway used by cancer cells. Since both receptors phosphorylate the
Rapamycin has a biphasic effect on insulin sensitivity in C2C12 myotubes due to sequential disruption of mTORC1 and mTORC2.
Ye, L; Varamini, B; Lamming, DW; Sabatini, DM; Baur, JA
Frontiers in Genetics null
Kyle D Copps et al.
The Journal of biological chemistry, 291(16), 8602-8617 (2016-02-06)
Constitutive activation of the mammalian target of rapamycin complex 1 and S6 kinase (mTORC1→ S6K) attenuates insulin-stimulated Akt activity in certain tumors in part through "feedback" phosphorylation of the upstream insulin receptor substrate 1 (IRS1). However, the significance of this
Yunxue Guo et al.
International journal of biological sciences, 8(10), 1408-1417 (2012-12-01)
Generally, most miRNAs that were up-regulated during differentiation promoted adipogenesis, but our research indicated that up-regulation of miR-145 in porcine preadipocytes did not promote but inhibit adipogenesis. In this study, miR-145 was significantly up-regulated during porcine dedifferentiated fat (DFAT) cells
Nancy J Hançer et al.
The Journal of biological chemistry, 289(18), 12467-12484 (2014-03-22)
IRS1 and IRS2 are key substrates of the insulin receptor tyrosine kinase. Mass spectrometry reveals more than 50 phosphorylated IRS1 serine and threonine residues (Ser(P)/Thr(P) residues) in IRS1 from insulin-stimulated cells or human tissues. We investigated a subset of IRS1
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