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Analysis of urinary metabolites of [1, 2-3H]-aldosterone was performed in the male guinea-pig. Separation of urinary metabolites was carried out by countercurrent distribution followed by DEAE-Sephadex A-25 column chromatography. A major component was obtained which was both hydrolyzable with sulphatase
After a large amount of aldosterone was injected into a male rabbit, urine was collected for 48 h. Separation of urinary aldosterone metabolites into monoglucosiduronate fraction and monosulphate fraction was carried out by a combination of countercurrent distribution and DEAE-Sephadex
Metabolism: clinical and experimental, 42(4), 470-476 (1993-04-01)
The effect of uremia on hepatic metabolism of aldosterone was studied in the isolated perfused liver of female Wistar rats. Uremia was induced by five-sixths partial nephrectomy 4 weeks before experiments. Isolated livers of normal and uremic rats were perfused
Specific methods are described for the enzymatic synthesis of each of the six possible 3H-labeled Ring-A reduced metabolites of aldosterone (5 alpha- and 5 beta-DHAldo; 3 alpha,5 alpha-THAldo; 3 beta,5 alpha-THAldo; 3 alpha,5 beta-THAldo; and 3 beta,5 beta-THAldo; see footnote
Annales de biologie clinique, 45(1), 70-73 (1987-01-01)
The authors describe a method of titration of tetrahydroaldosterone in the urine, requiring an internal non-radioactive standard. This method uses high performance liquid chromatography as a mean of isolating tetrahydroaldosterone (THAldo) and chromatography in gaseous phase as a method of
Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.
