价格与库存信息目前不能提供
推荐产品
生物源
bovine pancreas
品質等級
種類
Type V-S
形狀
powder
比活性
≥8,500 BAEE units/mg protein (biuret)
UniProt登錄號
儲存溫度
−20°C
基因資訊
cow ... TRYP8(282603)
一般說明
The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-sulfonate binding.
應用
Trypsin has been used in a study to assess the ontogeny of the endocrine pancreas in the fetal/newborn baboon. [1] Trypsin digestion and hydrogen/deuterium exchange support the transition between inward- and outward-facing conformations during the catalytic cycle of the bacterial multidrug ATP-binding cassette transporter. [2]
生化／生理作用
Lys-acetylated trypsin is less susceptible to autolysis than the native form.
單位定義
One BAEE unit will produce a ΔA253 of 0.001 per min at pH 7.6 at 25° C using BAEE as substrate. Reaction volume = 3.2 mL (1 cm light path).
準備報告
Prepared by the method of Labouesse, J. and Gervais, M., Eur. J. Biochem., 2, 215 (1967).
其他說明
View more information on trypsin at www.sigma-aldrich.com/enzymeexplorer.
基底
抑制劑
产品编号
说明
价格
訊號詞
Danger
危險分類
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
標靶器官
Respiratory system
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
個人防護裝備
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
其他客户在看
R F Regan et al.
Journal of neurochemistry, 70(1), 77-85 (1998-01-09)
The effect of magnesium on the oxidative neuronal injury induced by hemoglobin was assessed in murine cortical cell cultures. Exposure to 5 microM hemoglobin in physiologic (1 mM) magnesium for 26 h resulted in the death of about one-half the
Shahid Mehmood et al.
Proceedings of the National Academy of Sciences of the United States of America, 109(27), 10832-10836 (2012-06-20)
The study of membrane proteins remains a challenging task, and approaches to unravel their dynamics are scarce. Here, we applied hydrogen/deuterium exchange (HDX) coupled to mass spectrometry to probe the motions of a bacterial multidrug ATP-binding cassette (ABC) transporter, BmrA
Amy R Quinn et al.
The Journal of endocrinology, 214(3), 289-299 (2012-06-23)
Erratic regulation of glucose metabolism including hyperglycemia is a common condition in premature infants and is associated with increased morbidity and mortality. The objective of this study was to examine histological and ultrastructural differences in the endocrine pancreas in fetal
Nongluk Sriwilaijaroen et al.
Methods in molecular biology (Clifton, N.J.), 1200, 107-120 (2014-08-15)
The correlation between precise interactions of influenza A virus hemagglutinins with host cell surface glycans having terminal sialic acids and host range specificity has provoked the development of a high-throughput viral-receptor specificity assay. Here, we describe the use of the
Shin-Ichi Nakakita et al.
Methods in molecular biology (Clifton, N.J.), 2132, 567-583 (2020-04-20)
We describe a method to detect influenza virus using an evanescent-field-activated fluorescence scanner type glycan array and ELISA system. Neoglycoprotein was prepared by combination of organic chemistry and biomaterial preparation. These ligands were spotted on a glass plate or plastic
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