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運輸包裝
dry ice
儲存溫度
−20°C
相关类别
一般說明
胰蛋白酶是一种丝氨酸蛋白酶，可特异性水解赖氨酸及精氨酸残基的羧基侧的多肽键。该款修饰后的胰蛋白酶经过甲苯磺酰基-L-氨基联苯氯甲基酮 (TPCK) 处理，可使外源性糜蛋白酶失活。每个包装含8个小瓶，每瓶含25 μg。
應用
TPCK处理的胰蛋白酶已被用于:
- 作为杜尔贝科改良伊格尔培养基（DMEM）的补充剂，用于上皮样猪肾细胞系（LLC-PK1） 的猪Delta冠状病毒（PDCoV）感染实验
- 分离人脐静脉内皮细胞（HUVEC） 用于膜联蛋白-V/碘化丙啶 (PI) 染色测定
- 用于多循环复制动力学的最小必需培养基 （MEM）
生化／生理作用
N-对-对甲苯磺酰基-L-苯丙氨酸氯甲基酮（TPCK） 可用作糜蛋白酶的不可逆抑制剂。胰蛋白酶诱导人纤维细胞分化。胰蛋白酶被广泛用于蛋白质组学中的蛋白质样品消化。在细胞培养中，进行胰蛋白酶消化是为了使贴壁细胞彼此分开并离开培养容器壁。
訊號詞
Danger
危險分類
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
標靶器官
Respiratory system
儲存類別代碼
10 - Combustible liquids
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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