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Merck

B3279

Sigma-Aldrich

Nα-苯甲酰-L-精氨酸-4-硝基苯胺 盐酸盐

proteolytic enzyme substrate, chromogenic, >98% (TLC), powder

别名:

L-BAPA, L-BAPNA, BANI

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About This Item

经验公式(希尔记法):
C19H22N6O4 · HCl
CAS号:
分子量:
434.88
Beilstein:
4081878
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
PubChem物質ID:
NACRES:
NA.32

product name

Nα-苯甲酰-L-精氨酸-4-硝基苯胺 盐酸盐, >98% (TLC)

品質等級

化驗

>98% (TLC)

形狀

powder

溶解度

acetone: water (1:1): 40.00-51.00 mg/mL, clear, colorless to yellow

儲存溫度

−20°C

SMILES 字串

Cl[H].NC(=N)NCCC[C@H](NC(=O)c1ccccc1)C(=O)Nc2ccc(cc2)[N+]([O-])=O

InChI

1S/C19H22N6O4.ClH/c20-19(21)22-12-4-7-16(24-17(26)13-5-2-1-3-6-13)18(27)23-14-8-10-15(11-9-14)25(28)29;/h1-3,5-6,8-11,16H,4,7,12H2,(H,23,27)(H,24,26)(H4,20,21,22);1H/t16-;/m0./s1

InChI 密鑰

DEOKFPFLXFNAON-NTISSMGPSA-N

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一般說明

Na-Benzoyl-L-arginine 4-nitroanilide hydrochloride (L-BAPNA) is a colorless, chromogenic substrate for proteolytic enzymes such as trypsin, actinidin, and papain.

應用

Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride has been used as substrate:
  • to measure papain activity in spectrophotometric amidase assay
  • to measure the inhibitory function of human cystatin C (HCC) on the protease activity of papain by colorimetric assay
  • for trypsin

基底

胰蛋白酶和其他蛋白水解酶的显色底物。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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A chemical approach for detecting sulfenic acid-modified proteins in living cells
Reddie KG, et al.
Molecular Biosystems, 4(6), 521- 531 (2008)
Efficient expression, purification and characterization of native human cystatin C in Escherichia coli periplasm
Zhou Y, et al.
Protein Expression and Purification, 111, 18- 22 (2015)
Isolation, expression and characterization of a novel dual serine protease inhibitor, OH-TCI, from king cobra venom
He YY, et al.
Peptides, 29(10), 1692- 1699 (2008)
Daniele O B Sousa et al.
Nutrients, 7(7), 6038-6054 (2015-07-25)
The biochemical and nutritional attributes of two soybean (Glycine max (L.) Merr.) cultivars, one susceptible (Seridó) and the other resistant (Seridó-RCH) to stem canker, were examined to assess whether the resistance to pathogens was related to levels of antinutritional and/or
Raquel O Rocha et al.
Journal of agricultural and food chemistry, 63(22), 5335-5343 (2015-06-03)
Several studies have described the effects of seed exudates against microorganisms, but only few of them have investigated the proteins that have defensive activity particularly against nematode parasites. This study focused on the proteins released in the exudates of soybean

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