RIP
Imprint® RNA免疫沉淀试剂盒
High-capacity Protein A magnetic beads for successful RNA Immunoprecipitation,suitable for use with mRNA and microRNA
别名:
Magnetic Bead RNA Immunoprecipitation, RNA Immunopreciptation, mRNA Immunopreciptation, microRNA Immunoprecipitation
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About This Item
UNSPSC代码:
41116158
NACRES:
NA.54
推荐产品
一般描述
RNA免疫沉淀（RIP）是研究核糖核蛋白（RNP）复合物中RNA结合蛋白（RBP）及其RNA靶点的强大方法。抗特定RBP的抗体被用于共沉淀RNP，即RBP及其RNA伴侣。然后，可以通过下一代测序鉴定RNA或通过RT-PCR检测特定RNA。
应用
Imprint® RNA免疫沉淀试剂盒已可用于：
- 适用于下游应用
- 从个体靶标表征到全基因组图谱分析技术
- 信号转导途径的表征
- 验证ChIp-chIP和ChIP-seq数据
特点和优势
- 更高结合能力的Xtra蛋白A磁珠
- 细胞裂解和免疫沉淀的分步实验方案
- 包含RNA酶抑制剂和无RNA酶试剂，以保持RNA的完整性
- 内附阴性对照，且包含抗体
法律信息
Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany
试剂盒组分也可单独购买
产品编号
说明
化学品安全说明书
- I5381IgG from mouse serum, reagent grade, ≥95% (SDS-PAGE), lyophilized powder化学品安全说明书
- I5006IgG from rabbit serum, reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powder化学品安全说明书
- M7023Anti-Mouse IgG (whole molecule) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution化学品安全说明书
- P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution化学品安全说明书
- R1158Ribonuclease Inhibitor, recombinant, expressed in E. coli, 20-40 units/μL化学品安全说明书
自备
产品编号
说明
价格
警示用语：
Warning
危险分类
Aquatic Chronic 3 - Eye Irrit. 2 - Flam. Liq. 3 - Skin Irrit. 2
储存分类代码
3 - Flammable liquids
WGK
WGK 3
闪点(°F)
100.4 °F
闪点(°C)
38 °C
其他客户在看
Huijuan Xiao et al.
Cell & bioscience, 8, 23-23 (2018-03-29)
The acquisition of drug resistance has been considered as a main obstacle for cancer chemotherapy. Tumor protein 53 target gene 1 (TP53TG1), a p53-induced lncRNA, plays a vital role in the progression of human cancers. However, little is known about
Lin Cao et al.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 93, 570-579 (2017-07-08)
Non-small cell lung cancer (NSCLC), known as the most common type of lung cancer, has caused great economic losses and brought the patients with NSCLC great suffering. The NSCLC chemo-resistance to cisplatin (DDP) -based chemotherapy remains a huge challenge. The
Nicola Amodio et al.
Leukemia (2018-03-01)
The biological role and therapeutic potential of long non-coding RNAs (lncRNAs) in multiple myeloma (MM) are still to be investigated. Here, we studied the functional significance and the druggability of the oncogenic lncRNA MALAT1 in MM. Targeting MALAT1 by novel
Qingling Yuan et al.
The international journal of biochemistry & cell biology, 98, 1-9 (2018-02-24)
Papillary thyroid carcinoma (PTC) is the most common thyroid malignancy. Besides, increasing evidence has demonstrated that long non-coding RNA (lncRNA) HOTTIP played a crucial role in cancer pathogenesis. MiR-637-mediated Akt1 was involved in cell growth, invasion and migration in various
Zhi-Jun Zhu et al.
Biochemical and biophysical research communications, 497(4), 971-977 (2018-02-11)
Long non-coding RNAs are critically involved in oncogenesis in various cancer types. Here we reported a novel lncRNA signature correlated with progression of non-small cell lung cancer (NSCLC). In particular, we identified elevated expression of terminal differentiation-induced noncoding RNA (TINCR)
商品
Sigma’s Imprint RNA Immunoprecipitation Kit was used to copurify human argonaute 2 (Ago2)-associated RNAs from HeLa cells. MicroRNAs reverse transcribed and quantitating using Mysticq reagents.
Sigma Imprint RNA 免疫沉淀试剂盒用于从 HeLa 细胞中共纯化人 argonaute 2 (Ago2) 相关 RNA。MicroRNA通过Mysticq 试剂进行逆转录和定量。
实验方案
Procedure and protocol for Anti Ago-RNA Immunoprecipitation from mammalian cells using the RIP kit
使用RIP试剂盒进行哺乳动物细胞Ago抗体-RNA免疫沉淀的实验方案和流程
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