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S4438

Sigma-Aldrich

SYBR® Green JumpStart™ Taq ReadyMix™

for quantitative PCR, MgCI2 in buffer

别名:

Hot start PCR master mix, sybr green PCR master mix, sybr green qPCR

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About This Item

MDL號碼:
MFCD03458666
分類程式碼代碼:
41106300
NACRES:
NA.55

品質等級

200

形狀

liquid

用途

sufficient for 100 reactions
 sufficient for 20 reactions
 sufficient for 500 reactions

特點

dNTPs included
hotstart

濃度

1.25 units/reaction (50 μL reaction volume)

技術

qPCR: suitable

輸入

purified DNA

適合性

suitable for (quantitative PCR)

應用

agriculture

相容性

ABI 7000
 ABI 7300
 ABI 7500 Fast
 ABI 7500
 ABI 7700
 ABI 7900 HT Fast
 ABI 7900 HT
 ABI 7900
 ABI StepOne
 ABI StepOnePlus
 ABI ViiA 7
Bio-Rad CFX384
 Bio-Rad CFX96
 Bio-Rad MJ Chromo4
 Bio-Rad MJ Opticon 2
Bio-Rad MJ Opticon
 Bio-Rad MiniOpticon
 Bio-Rad MyiQ
 Bio-Rad iCycler iQ
 Bio-Rad iQ 5
 Cepheid SmartCycler
 Eppendorf® Mastercycler ep realplex
Eppendorf® Mastercycler ep realplex2 S
 Illumina Eco qPCR
 Qiagen Corbett Rotor-Gene 3000
 Qiagen Corbett Rotor-Gene 6000
 Qiagen Corbett Rotor-Gene Q
 Roche LightCycler 480
Strategene Mx3000P
 Strategene Mx3005P
 Strategene Mx4000
for use with ABI 5700

檢測方法

SYBR® Green

運輸包裝

wet ice

儲存溫度

−20°C

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相关类别

一般說明

SYBR® Green I是定量任意DNA序列的理想之选。这种染料可与双链DNA结合，并可通过测定整个循环的荧光增量监测其检出结果。
SYBR® Green JumpStart ™Taq ReadyMix™将JumpStart Taq抗体用于热启动SYBR GreenI PCR高效表现和ReadyMix溶液的方便性结合在一起。我们提供的即用型SYBR GreenI，JumpStart Taq DNA聚合酶，99%纯脱氧核糖核酸和反应缓冲液的混合物是2X浓度，可以方便使用。直接将2X25μL混合物加入DNA模板、引物和水中。JumpStart Taq抗体在室温下能够使DNA聚合酶失活。当温度在第一步循环变性中升高到70℃以上，复合体解离，聚合酶完全被激活。JumpStart Taq DNA聚合酶防止非特异性扩增，能够获得更为准确的CT值。

應用

SYBR® Green JumpStart™ Taq ReadyMix™已用于：

  • 定量PCR (qPCR)，从髓鞘碱性蛋白(mbp)的所有变体扩增单一保护区SRY-Box 10 (sox10)，以及β-肌动蛋白基因
  • 实时荧光定量PCR，检测每个扩增周期的PCR产物的累积情况
  • DNA模板和cDNA模板的常规qPCR扩增
  • 通过实时定量逆转录-PCR (qRT-PCR)扩增RNA

特點和優勢

  • 通过在ReadyMix中进行SYBR Green检测提供抗体灭活热启动PCR的优势，是高通量应用的理想之选；只需引物和模板即可
  • JumpStart™ Taq DNA聚合酶可防止非特异性产物扩增，进而提高效率和靶标得率。
  • SYBR® Green JumpStart® Taq ReadyMix适用于基于SYBR的qPCR，采用MgCl2配制或采用独立小瓶包装，便于优化。ReadyMixes与管式和板式仪器兼容
  • 该预混液可确保反应间一致性
  • 可最大限度减少污染
  • 减少了引物二聚体
  • 与手动或蜡质热启动®方法相比，制备时间更短
  • 允许室温构建反应并随附荧光染料；qPCR应用的理想之选

包裝

默认反应体积为50 μL

20RXN包装为1 X 500μL
100RXN包装为1 X 2.5 mL
400RXN包装为1 X 10 mL
500RXN包装为1 X 12.5 mL

原則

SYBR Green JumpStart Taq ReadyMix可用于单个产品实时扩增，也可以用于产生荧光标记探针之前的PCR优化。 不建议将荧光标记探针与SYBR Green I染料一起使用。

SYBR Green I是一种常用的荧光DNA结合染料，可结合所有双链DNA，并通过测量整个循环中荧光的增加来监测检测。SYBR Green I最大激活和发射峰值分别在494nm和521nm。 ROX参考染料仪器设定符合定量PCR参考染料测量需要。 在加入一种热启动介导tag聚合酶，JumpStart Taq后，Sigma′s基于SYBR QPCR检测的特异性被增强。

法律資訊

本产品的使用受到如下一项或多项美国专利及其对应的境外专利声明保护：5,994,056和6,171,785。购买本产品，即相当于依照境外的专利声明获得了一份受限制、不可转让的使用许可，即将此等数量的产品用于购买者′内部的研究用途。未通过明示、暗示或禁止反言的形式授予任何其他专利要求书项下的权利（例如，美国专利号6,814,934中的设备或系统要求）及提供任何形式商业服务的权利，包括但不限于出于经济利益或其他商业考虑报告购买者的活动结果。′本品仅供研究使用。 如需用于Roche专利许可的诊断用途，需另外征得Roche许可。有关购买许可的更多信息，可联系许可总监（Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA）获取。
Eppendorf is a registered trademark of Eppendorf AG
HOTSTART is a registered trademark of Molecular BioProducts, Inc.
JumpStart is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Life Technologies

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象形圖

Exclamation markEnvironment
GHS07,GHS09

訊號詞

Warning

危險分類

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

儲存類別代碼

10 - Combustible liquids

閃點（°F）

Not applicable

閃點（°C）

Not applicable

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T Y C Pang et al.
Neuroscience, 141(2), 569-584 (2006-05-24)
Huntington's disease is a fatal neurodegenerative disorder caused by a mutation of the huntingtin gene and involves progressive motor abnormalities (including chorea), cognitive deficits (dementia) as well as psychiatric symptoms. We have previously demonstrated that environmental enrichment slows the onset
Blair R G Gordon et al.
Journal of bacteriology, 190(21), 7052-7059 (2008-09-09)
Lsr2 is a small, basic protein present in Mycobacterium and related actinomycetes. Our previous in vitro biochemical studies showed that Lsr2 is a DNA-bridging protein, a property shared by H-NS-like proteins in gram-negative bacteria. Here we present in vivo evidence
Bikem Soygur et al.
Human reproduction (Oxford, England), 31(7), 1455-1461 (2016-05-14)
As Syncytin 1 (human endogenous retrovirus (HERV-W)) is crucial for human embryo placentation is it expressed during preimplantation embryo development? Syncytin 1 was expressed mainly in trophoblast cells of the blastocyst particularly in cells underlying the inner cell mass (ICM).
Karim Sorefan et al.
Nature, 459(7246), 583-586 (2009-05-30)
Local hormone maxima are essential for the development of multicellular structures and organs. For example, steroid hormones accumulate in specific cell types of the animal fetus to induce sexual differentiation and concentration peaks of the plant hormone auxin direct organ
Mathew S Box et al.
Plant methods, 7, 7-7 (2011-03-15)
Many experiments in modern plant molecular biology require the processing of large numbers of samples for a variety of applications from mutant screens to the analysis of natural variants. A severe bottleneck to many such analyses is the acquisition of
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