跳转至内容
Merck
所有图片(1)

Key Documents

View All Documentation

O4387

Sigma-Aldrich

Oligo(dT)23, 锚定

70 μM in H2O

别名:

Oligo(dT)23锚定引物

登录查看公司和协议定价
所有图片(1)

About This Item

分類程式碼代碼:
41106305
NACRES:
NA.52

生物源

synthetic (organic)

品質等級

200

形狀

solution

用途

0.1 mL sufficient for 100 RT-PCR reactions (as described in the Technical Bulletin for Product Codes HSRT100 and HSRT20)

濃度

70 μM in H2O

顏色

colorless

運輸包裝

wet ice

儲存溫度

−20°C

相关类别

一般說明

锚定寡核苷酸(dT)23引物用于启动聚(A)尾的mRNA，以进行cDNA合成。引物有23个胸腺嘧啶残基，在3′端有一个G、C或A残基（锚点）。这个锚点可确保寡核苷酸(dT)引物在信使的起点结合，并且没有很长的不可用序列区域。增强型AMV逆转录酶、模板RNA、引物的最佳浓度和扩增参数取决于所使用的系统，并且应该根据经验确定。

應用

锚定寡核苷酸(dT)23已用于实时定量聚合酶链反应（PCR）的cDNA合成。
锚定寡核苷酸23 引物由23个胸腺嘧啶核苷残基组成，并在3′端具有一个G、C或A残基（锚）。该锚定位点可确保寡核苷酸(dT)23引物与mRNA起始端结合，直接跳过无用的长序列区域。以poly(A)+ RNA为模板制备cDNA时，该锚定寡核苷酸 (dT)23 引物比标准寡核苷酸 (dT) 引物更具优势。

特點和優勢

  • 在制备cDNA文库过程中，当序列信息不完整或缺失或者特异性引物不合适时，可以使用Oligo(dT)23锚定引物和随机九聚体（产品编号R 7647）。
  • 这些引物可以在cDNA第一链合成、cDNA文库构建和各种其他应用中作为逆转录引物的替代品。
  • 转录后，oligo(dT)23锚定引物在高温（高达65 °C）下启动引物的能力降低，避免干扰PCR。
  • 由poly(A)+ RNA产生cDNA时，oligo(dT)23锚定引物优于oligo(dT)标准引物。

其他說明

仅供实验室使用。不可用于药物、家庭或其他用途。

儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

Leila Azimi et al.
Gene, 576(1 Pt 1), 166-170 (2015-10-13)
Carbapenemase production causes multi antibiotics resistant in Gram-negative bacteria. A simple rapid and accurate phenotypic test for detection of Gram-negative carbapenemase-producing bacteria is useful for the treatment of infections. The aim of this study was to track the negative results
Fiona S McDonnell et al.
Journal of glaucoma, 25(10), e834-e842 (2016-10-18)
Glaucoma is an optic neuropathy that affects 60 million people worldwide. There is an underlying fibrosis associated with the lamina cribrosa (LC) in glaucoma. DNA methylation is well established in regulating fibrosis and may be a therapeutic target for glaucoma.
Sigma's New Enhanced Avian RT-PCR Kit.
Eastlund, E. and Song, K.
Sigma data, 1, 15-17 (2000)
Andrea E Vasconez et al.
Acta physiologica (Oxford, England), 225(1), e13168-e13168 (2018-08-05)
The histone demethylase Jarid1b limits gene expression by removing the active methyl mark from histone3 lysine4 at gene promoter regions. A vascular function of Jarid1b is unknown, but a vasoprotective function to inflammatory and hypertrophic stimuli, like angiotensin II (AngII)
Christian Fork et al.
Journal of lipid research, 58(2), 386-392 (2016-12-04)
Nonsteroidal anti-inflammatory drugs are the most widely used medicine to treat pain and inflammation, and to inhibit platelet function. Understanding the expression regulation of enzymes of the prostanoid pathway is of great medical relevance. Histone acetylation crucially controls gene expression.
查看所有相关文献

商品

Reverse Transcription

Challenges in gene expression analysis include mRNA stability, temporal transcription patterns, and mRNA-protein correlation, impacting accuracy.

Reverse Transcription

Challenges in gene expression analysis include mRNA stability, temporal transcription patterns, and mRNA-protein correlation, impacting accuracy.

Reverse Transcription

Challenges in gene expression analysis include mRNA stability, temporal transcription patterns, and mRNA-protein correlation, impacting accuracy.

逆转录

分析基因表达的方法之一是测量基因的mRNA浓度。此类分析面临着若干挑战，例如不同转录本之间的半衰期不同、转录时间模式以及mRNA和蛋白质之间缺乏相关性。

查看所有结果

实验方案

The 3'/5' Assay for Analysis of RNA Integrity Protocol

The 3’/5’ integrity assay identifies RNA degradation, crucial for large sample sets or less detectable degradation.

The 3'/5' Assay for Analysis of RNA Integrity Protocol

The 3’/5’ integrity assay identifies RNA degradation, crucial for large sample sets or less detectable degradation.

The 3'/5' Assay for Analysis of RNA Integrity Protocol

The 3’/5’ integrity assay identifies RNA degradation, crucial for large sample sets or less detectable degradation.

The 3'/5' Assay for Analysis of RNA Integrity Protocol

The 3’/5’ integrity assay identifies RNA degradation, crucial for large sample sets or less detectable degradation.

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门